| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LYVE1 |
| Entrez GeneID | 10894; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.271~275 (K-N-Q-Q-K) derived from Human Lymphatic vessel endothelial hyaluronic acid receptor 1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于LYVE-1抗体的关键文献摘要：
1. **文献名称**：*LYVE-1. a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan*
**作者**：Banerji S, et al. (1999)
**摘要**：首次克隆并鉴定了LYVE-1分子，证明其特异性表达于淋巴管内皮细胞，并作为透明质酸受体，提出LYVE-1抗体可作为淋巴管标记物。
2. **文献名称**：*LYVE-1 marks lymphatic vessels in human tumours but is absent from the blood vasculature*
**作者**：Prevo R, et al. (2001)
**摘要**：通过LYVE-1抗体免疫组化分析，证实其在多种人类肿瘤淋巴管内皮中的特异性表达，而血管内皮无此标记，支持其在肿瘤淋巴管研究中的应用。
3. **文献名称**：*The Lymphatic System: A New Frontier in Metastasis Research*
**作者**：Jackson DG (2003)
**摘要**：综述中强调LYVE-1抗体在区分淋巴管与血管中的关键作用，并讨论其在肿瘤转移机制研究中的价值。
4. **文献名称**：*LYVE-1-positive macrophages are present in normal and cancer-associated non-lymphatic tissues*
**作者**：Kerjaschki D, et al. (2006)
**摘要**：研究发现LYVE-1抗体不仅标记淋巴管，还可染色部分组织巨噬细胞，提示需谨慎解释其在复杂微环境中的染色结果。
以上文献为LYVE-1抗体的基础研究和应用提供了关键依据。
The Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1 (LYVE-1) antibody is a widely used tool for identifying lymphatic endothelial cells in biological research. LYVE-1. a transmembrane glycoprotein, serves as a receptor for hyaluronic acid (HA) and belongs to the Link protein superfamily. Structurally, it shares homology with CD44 but exhibits distinct ligand-binding properties and tissue distribution. Primarily expressed on lymphatic vessel endothelial cells, LYVE-1 plays roles in lymphatic development, HA homeostasis, and immune cell trafficking. Its expression is also observed in specialized vascular beds, such as liver sinusoidal endothelial cells, and subsets of tissue-resident macrophages, though functional significance in these contexts remains under investigation.
LYVE-1 antibodies are critical for visualizing lymphatic networks in tissues via techniques like immunohistochemistry, immunofluorescence, and flow cytometry. They enable studies on lymphangiogenesis in pathological conditions, including cancer metastasis, chronic inflammation, and lymphedema. Researchers utilize these antibodies to assess lymphatic vessel density or integrity in experimental models, providing insights into disease mechanisms. However, variability in LYVE-1 expression across species (e.g., stronger in mice vs. humans) and contexts (e.g., downregulation in tumor-associated lymphatics) requires careful experimental validation. Commercial LYVE-1 antibodies are typically raised against conserved epitopes, with clones like ALY7 and 103PA2 commonly employed. While highly specific for lymphatic endothelium in most tissues, cross-reactivity with HA-rich extracellular matrices or non-lymphatic cell types necessitates appropriate controls.
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