| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MERL; NF2; Neurofibromin 2; SCH; Schwannomerlin |
| Entrez GeneID | 4771; |
| WB Predicted band size | 69kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.516~520 (R-L-S-M-E) derived from Human Merlin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Merlin (Ab-518)抗体的3篇参考文献示例(注：Ab-518为假设性抗体编号，以下内容为模拟生成，仅供参考)：
---
1. **标题**: "Merlin/NF2 phosphorylation regulates cell adhesion and apoptosis in mesothelioma"
**作者**: Smith J, et al.
**摘要**: 本研究利用Merlin抗体(Ab-518)通过Western blot和免疫荧光技术，验证了Merlin蛋白在间皮瘤细胞中的磷酸化状态及其对细胞黏附的调控作用，发现磷酸化缺陷导致Merlin肿瘤抑制功能丧失。
2. **标题**: "Antibody validation for NF2-associated schwannoma studies"
**作者**: Lee H, et al.
**摘要**: 文章系统评估了多种Merlin抗体的特异性，包括Ab-518.通过siRNA敲低实验和免疫组化证实，Ab-518在石蜡包埋组织中能特异性识别Merlin C端结构域，适用于神经鞘瘤的病理学分析。
3. **标题**: "Role of Merlin in Hippo signaling pathway"
**作者**: Garcia R, et al.
**摘要**: 使用Ab-518抗体进行共聚焦显微镜分析，发现Merlin与Hippo通路核心蛋白YAP的相互作用依赖于细胞密度变化，揭示了其在肿瘤抑制中的动态调控机制。
---
**注意**：若需真实文献，建议通过PubMed或抗体生产商官网(如CST、Abcam)查询具体抗体编号(如Ab-518可能为品牌定制编号)的相关引用文献。
Merlin (Moesin-Ezrin-Radixin-like protein), encoded by the NF2 gene, is a tumor suppressor protein critical for regulating cell growth, adhesion, and motility. It belongs to the FERM (Four-point-one, Ezrin, Radixin, Moesin) protein family, which links membrane proteins to the cytoskeleton. Merlin functions by controlling signaling pathways such as Hippo, PI3K/AKT, and MAPK, thereby maintaining tissue homeostasis and inhibiting tumorigenesis. Mutations in the NF2 gene are associated with neurofibromatosis type 2 (NF2), a hereditary disorder characterized by benign nervous system tumors, as well as sporadic cancers like mesothelioma and schwannoma.
The Merlin (Ab-518) antibody is a monoclonal antibody specifically designed to detect endogenous Merlin protein levels in various experimental applications, including Western blotting, immunohistochemistry, and immunofluorescence. It recognizes a conserved epitope, enabling reliable specificity across human and rodent samples. Researchers utilize this antibody to investigate Merlin's expression patterns, post-translational modifications (e.g., phosphorylation at serine 518), and interactions with binding partners in normal and diseased tissues. Its role in elucidating Merlin's tumor-suppressive mechanisms—particularly in cytoskeletal organization, contact inhibition, and pathway regulation—makes it a vital tool for studying cancer biology and developing therapeutic strategies targeting NF2-related pathologies.
×
