Rabbit Polyclonal cdc25A(Ab-76) Antibody

The CDC25A (Ab-76) antibody is a monoclonal antibody specifically designed to target the CDC25A protein, a dual-specificity phosphatase critical for cell cycle regulation. CDC25A facilitates G1/S and G2/M phase transitions by dephosphorylating and activating cyclin-dependent kinases (CDKs), such as CDK2 and CDK1. Dysregulation of CDC25A is implicated in uncontrolled cell proliferation, genomic instability, and cancer progression, making it a focus in oncological research. The Ab-76 clone recognizes an epitope near the N-terminal region of human CDC25A, often detecting both phosphorylated and unmodified forms, depending on experimental conditions. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study CDC25A expression, localization, and post-translational modifications in cell lines or tissues. Studies employing this antibody have elucidated CDC25A’s role in DNA damage responses, where its degradation is triggered to halt cell cycle progression. Additionally, it has been utilized to explore CDC25A overexpression in cancers, including breast and colorectal tumors, linking it to poor prognosis and therapeutic resistance. Validation typically includes testing in CDC25A knockout models to confirm specificity. Researchers value this antibody for its reliability in probing CDC25A dynamics in both physiological and pathological contexts.