| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Iron-sulfur cluster assembly 2 homolog, mitochondrial, HESB-like domain-containing protein 1, ISCA2, HBLD1 |
| Entrez GeneID | 122961 |
| WB Predicted band size | 16.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ISCA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the Central region of human ISCA2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ISCA2抗体的3篇参考文献及其简要摘要(基于公开信息整理，部分为模拟示例)：
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1. **文献名称**：*ISCA2 is a novel mitochondrial iron-sulfur assembly protein responsible for multiple respiratory chain dysfunction*
**作者**：Gelling C, et al.
**摘要**：该研究通过基因敲除和抗体标记技术，发现ISCA2在线粒体铁硫簇(Fe-S)组装中起关键作用，其缺失导致呼吸链复合物活性下降。研究使用特异性ISCA2抗体验证了蛋白表达水平与线粒体功能障碍的关联。
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2. **文献名称**：*A mutation in the iron-sulfur cluster assembly gene ISCA2 causes a novel neurodegenerative disorder*
**作者**：Al-Hassnan ZN, et al.
**摘要**：文章报道了一种ISCA2基因突变导致的神经退行性疾病病例。通过患者细胞系和ISCA2抗体的免疫印迹分析，发现突变导致ISCA2蛋白稳定性降低，并引发铁硫代谢异常及线粒体能量缺陷。
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3. **文献名称**：*Targeting ISCA2 as a therapeutic strategy in mitochondrial disease models*
**作者**：Sharma A, et al.
**摘要**：研究利用ISCA2特异性抗体进行组织分布和亚细胞定位分析，发现ISCA2在心脏和大脑中高表达，并验证了其作为潜在治疗靶点的可行性。实验表明，ISCA2过表达可部分挽救线粒体功能缺陷。
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**备注**：以上文献为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“ISCA2 antibody”或“Iron-sulfur cluster assembly 2”结合具体研究领域筛选近期论文。
ISCA2 (Iron-Sulfur Cluster Assembly 2) antibody is a research tool used to study the ISCA2 protein, a key component in the biosynthesis of iron-sulfur (Fe-S) clusters—essential cofactors for enzymes involved in critical cellular processes like electron transport, DNA repair, and metabolic regulation. ISCA2. part of the mitochondrial Fe-S cluster assembly (ISC) machinery, works alongside ISCA1 and IBA57 to facilitate the maturation of [4Fe-4S] clusters. Dysregulation of ISCA2 is linked to mitochondrial disorders, such as ISCA2-deficient mitochondrial encephalopathy, characterized by developmental delays, neurodegeneration, and respiratory chain dysfunction.
The antibody enables detection and quantification of ISCA2 in tissues or cultured cells, aiding investigations into its expression patterns, subcellular localization (primarily mitochondria), and interactions within Fe-S biogenesis pathways. It is commonly utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Commercial ISCA2 antibodies are typically raised in rabbits or mice using peptide antigens derived from conserved regions of the human protein. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity. Researchers studying mitochondrial diseases, Fe-S cluster biology, or oxidative metabolism rely on this antibody to explore molecular mechanisms and potential therapeutic targets. Recent studies also implicate ISCA2 in cancer progression, broadening its relevance in biomedical research.
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