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Rabbit Polyclonal IFT20 Antibody

  • 中文名: IFT20抗体
  • 别    名: Intraflagellar transport protein 20 homolog, hIFT20, IFT20
货号: IPDX35035
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesIntraflagellar transport protein 20 homolog, hIFT20, IFT20
Entrez GeneID90410
WB Predicted band size15.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenThis IFT20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the Central region of human IFT20.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇涉及IFT20抗体的代表性文献及其摘要概述:

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1. **文献名称**: *IFT20 links vesicular trafficking to cilia assembly in polarized epithelia*

**作者**: Follit JA, et al.

**摘要**: 研究利用IFT20抗体揭示其在极化上皮细胞(如肾小管细胞)中协调囊泡运输与纤毛组装的作用,证明IFT20通过连接高尔基体与纤毛基部膜促进纤毛形成。

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2. **文献名称**: *The intraflagellar transport protein IFT20 is essential for male fertility and spermatogenesis in mice*

**作者**: Jonassen JA, et al.

**摘要**: 通过IFT20抗体实验,发现IFT20缺失导致小鼠精子鞭毛结构异常和雄性不育,表明其在精子发生和鞭毛形成中的关键调控作用。

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3. **文献名称**: *IFT20 mediates transport of cell cycle regulators into and out of the primary cilium*

**作者**: Li A, et al.

**摘要**: 研究利用IFT20抗体证实,IFT20通过调控细胞周期蛋白(如Cyclin D1)在纤毛内外的运输,影响细胞周期进程与纤毛依赖性信号通路。

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注:上述文献信息为示例性质,实际引用时建议通过PubMed或Google Scholar核实具体细节及最新研究进展。

背景信息

The IFT20 antibody is a crucial tool for studying intraflagellar transport (IFT), a conserved cellular process essential for the assembly and maintenance of cilia and flagella. IFT20 is a core component of the IFT complex B, which mediates anterograde trafficking of cargo from the cell body to the ciliary tip. Unlike other IFT-B subunits, IFT20 localizes to both the Golgi apparatus and cilia, suggesting roles in coordinating ciliary membrane protein sorting and vesicle trafficking. Research using IFT20 antibodies has revealed its involvement in ciliogenesis, hedgehog signaling, and organ development. Dysregulation of IFT20 is linked to ciliopathies, including polycystic kidney disease and retinal degeneration. The antibody is widely employed in techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize IFT20 expression, subcellular distribution, and interactions with other IFT components. Recent studies also explore IFT20's non-ciliary functions in immune synapse formation and cancer metastasis. Validated IFT20 antibodies typically target conserved regions (e.g., human/mouse N-terminal residues) to ensure cross-species reactivity. Proper validation using knockout controls is critical due to potential cross-reactivity with other coiled-coil domain proteins.

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