Rabbit Polyclonal MYL2 Antibody

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     All lanes : Anti-MYL2 Antibody (Center) at 1:2000 dilution Lane 1: Human heart lysate Lane 2: Human skeletal muslce lysate Lane 3: Mouse heart lysate Lane 4: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing U-2 OS cells stained with P34486(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34486,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using P34486 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0).  Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded human heart tissue using P34486 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0).  Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature.  A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded Human Myocardium section using Pink1(Cat#P34486). P34486 was diluted at 1:250 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.    

MYL2 (myosin light chain 2) is a regulatory protein component of myosin, a molecular motor critical for muscle contraction. Specifically, MYL2 encodes the ventricular/atrial isoform of myosin regulatory light chain, predominantly expressed in cardiac and skeletal muscles. It binds to the head region of myosin heavy chains, modulating ATPase activity and influencing the mechanical function of sarcomeres. Dysregulation of MYL2 is linked to cardiomyopathies, particularly hypertrophic cardiomyopathy (HCM), where mutations in MYL2 can disrupt contractility and contribute to pathological cardiac hypertrophy.

MYL2 antibodies are essential tools in cardiovascular research, enabling the detection and quantification of MYL2 protein in tissues or cell lysates. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study MYL2 expression patterns, localization, and interactions in both normal and diseased states. Commercial MYL2 antibodies are typically raised against recombinant or peptide antigens, validated for specificity across species (human, mouse, rat). Their applications extend to investigating molecular mechanisms of heart diseases, evaluating MYL2 as a potential biomarker, and developing therapeutic strategies targeting myosin-associated pathways.