Rabbit Polyclonal CKS2 Antibody

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     All lanes : Anti-CKS2 Antibody (C-Term) at 1:2000 dilution Lane 1: Caco-2 whole cell lysate Lane 2: RPMI 8226 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed,  0. 1% Triton X-100 permeabilized A549 cells labeling CKS2 with P34485 at 1/25 dilution,  followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green).  Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing A549 cells stained with P34485(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34485,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

**Background of CKS2 Antibody**

The CKS2 (Cyclin-Dependent Kinase Regulatory Subunit 2) antibody is a tool used to detect and study the CKS2 protein, a member of the conserved CKS family involved in cell cycle regulation. CKS2 binds to cyclin-dependent kinases (CDKs), particularly CDK1 and CDK2. facilitating their interaction with cyclins to drive cell cycle progression, notably during the G1/S and G2/M transitions.

CKS2 is overexpressed in various cancers (e.g., colorectal, breast, and lung cancers) and is linked to tumorigenesis, poor prognosis, and chemotherapy resistance. Its role in promoting cell proliferation and genomic instability makes it a potential therapeutic target.

The CKS2 antibody is utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression levels, subcellular localization, and interactions in both normal and pathological contexts. Polyclonal and monoclonal variants target specific epitopes, with validation often including knockout controls to confirm specificity.

Research using CKS2 antibodies has highlighted its involvement in DNA damage repair, apoptosis evasion, and stemness maintenance in cancer cells. Understanding CKS2 dynamics aids in unraveling cell cycle dysregulation mechanisms in diseases, offering insights for diagnostic and therapeutic strategies.