Rabbit Polyclonal CANX Antibody

+ +

•  

   
     All lanes : Anti-CANX Antibody (C-Term) at 1:2000 dilution Lane 1: PANC-1 lysate Lane 2: Mouse brain lysate Lane 3: Mouse liver lysate Lane 4: Rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Overlay histogram showing HeLa cells stained with P34477(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34477,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

•  

   
     P34477 staining CANX in human hepatocarcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

CANX (Calnexin) is a key endoplasmic reticulum (ER)-resident chaperone protein critical for protein folding and quality control. It primarily assists in the structural maturation of newly synthesized glycoproteins by binding to monoglucosylated N-linked glycans via its lectin domain. CANX also interacts with the ER oxidoreductase ERP57 to facilitate disulfide bond formation. As a component of the calnexin/calreticulin cycle, it ensures proper folding of proteins entering the secretory pathway, retaining misfolded proteins for ER-associated degradation (ERAD).

CANX antibodies are widely used in research to study ER stress, protein trafficking, and cellular responses to unfolded proteins. These antibodies enable detection of CANX in techniques like Western blotting, immunofluorescence, and immunoprecipitation, serving as an ER marker in subcellular localization studies. They also help investigate CANX's role in diseases, including cancer (where overexpression is linked to metastasis), neurodegenerative disorders (e.g., Alzheimer’s), and viral infections (e.g., HCV, HIV), where viral proteins exploit CANX for replication. Commercially available CANX antibodies are typically raised against conserved epitopes, with host species including mouse, rabbit, and rat. Validation often involves knockout cell lines to confirm specificity. Researchers rely on these tools to explore ER homeostasis, adaptive stress pathways, and therapeutic targets related to proteostasis imbalances.