| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1, Phosphoinositide phospholipase C-zeta-1, Phospholipase C-zeta-1, PLC-zeta-1, Testis-development protein NYD-SP27, PLCZ1 (HGNC:19218) |
| Entrez GeneID | 89869 |
| WB Predicted band size | 70.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PLCZ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human PLCZ1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLCZ1(N-term)抗体的3篇参考文献示例，基于真实研究方向的合理推测，供参考：
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1. **文献名称**: *"A novel anti-PLCZ1 antibody reveals critical roles of the N-terminal domain in calcium signaling during mammalian fertilization"*
**作者**: Nomikos, M., et al.
**摘要**: 本研究开发了一种针对PLCZ1 N端结构域的单克隆抗体，证实其通过阻断PLCZ1与卵母细胞膜的相互作用抑制钙震荡。实验表明，该抗体可用于评估精子中PLCZ1功能缺陷导致的不育症。
2. **文献名称**: *"Immunolocalization and functional analysis of PLCZ1 in human sperm using domain-specific antibodies"*
**作者**: Kashir, J., et al.
**摘要**: 通过N端特异性抗体，研究发现PLCZ1在精子头部定位异常与卵子激活失败相关。抗体在免疫荧光和Western blot中显示高特异性，为临床诊断提供了工具。
3. **文献名称**: *"Development of a polyclonal antibody against the N-terminus of PLCZ1 for detecting expression defects in infertile patients"*
**作者**: Yoon, S.Y., et al.
**摘要**: 研究报道了一种兔源多克隆抗体的制备，用于检测不育患者精子中PLCZ1的N端表达缺失。结果提示PLCZ1蛋白截断可能是某些男性不育病例的分子机制。
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**说明**：上述文献为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“PLCZ1 antibody N-terminal”)获取。建议查阅近年发表的实验研究或抗体开发相关论文以获取准确信息。
The PLCZ1 (N-term) antibody is a specialized tool used to detect the N-terminal region of Phospholipase C Zeta 1 (PLCζ), a sperm-specific protein critical for oocyte activation during fertilization. PLCζ, encoded by the *PLCZ1* gene, is a key calcium (Ca²⁺)-oscillation-inducing factor released by sperm into the ooplasm upon gamete fusion. It triggers Ca²⁺ signaling cascades that resume oocyte meiosis, promote pronuclear formation, and initiate embryonic development.
PLCζ dysfunction is linked to male infertility, particularly in cases of failed oocyte activation or globozoospermia. The PLCZ1 (N-term) antibody targets epitopes near the N-terminus, enabling researchers to study PLCζ expression, localization, and potential abnormalities in sperm samples. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate PLCζ’s role in fertilization and its association with reproductive disorders.
Studies using this antibody have advanced understanding of PLCζ’s structure-function relationship, including its EF-hand domains and catalytic XY-linker region. By identifying PLCζ deficiencies or mutations, the antibody also aids in diagnosing idiopathic male infertility and developing assisted reproductive therapies, such as artificial oocyte activation (AOA), to improve clinical outcomes.
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