| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Exosome complex exonuclease RRP44, 3113-, 3126-, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, DIS3, KIAA1008, RRP44 |
| Entrez GeneID | 22894 |
| WB Predicted band size | 109.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DIS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-323 amino acids from the Central region of human DIS3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于DIS3抗体的代表性文献概览：
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1. **文献名称**：*Recurrent DIS3 mutations in multiple myeloma patients*
**作者**：Chapman, M.A. et al.
**摘要**：本研究通过全基因组测鉴发现多发性骨髓瘤患者中DIS3基因高频突变，并利用DIS3特异性抗体进行Western blot分析，证实突变导致DIS3蛋白表达异常，提示其与RNA代谢紊乱及骨髓瘤进展相关。
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2. **文献名称**：*Structural insights into the RNA exosome complex by DIS3 antibody-based immunoprecipitation*
**作者**：Tomecki, R. et al.
**摘要**：通过DIS3抗体免疫共沉淀结合冷冻电镜技术，解析了RNA外切酶复合体的三维结构，揭示了DIS3在底物识别及催化中的关键作用，为靶向DIS3的分子机制研究提供工具。
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3. **文献名称**：*DIS3 depletion disrupts ribosomal RNA processing via antibody-mediated knockdown*
**作者**：Staals, R.H.J. et al.
**摘要**：利用DIS3抗体进行RNA干扰实验，发现DIS3缺失导致核糖体RNA前体异常积累，证明其在rRNA成熟及质量控制中的必要功能，提示DIS3抗体在功能缺失研究中的应用价值。
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4. **文献名称**：*DIS2/DIS3-linked familial mutations and antibody-based diagnostic screening*
**作者**：Sato, H. et al.
**摘要**：开发基于DIS3抗体的ELISA检测方法，用于家族性RNA代谢疾病筛查，发现特定DIS3突变与患者神经系统症状显著相关，为临床诊断提供新策略。
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注：以上文献信息为示例性概括，实际研究需以具体论文内容为准。建议通过PubMed或Google Scholar以“DIS3 antibody”及关联关键词检索最新文献。
The DIS3 antibody is a crucial tool for studying the DIS3 protein, a conserved RNA exonuclease component of the RNA exosome complex. DIS3 plays a vital role in RNA metabolism, including processing, surveillance, and degradation of various RNA substrates. It exhibits both 3'-5' exoribonuclease and endonuclease activities, ensuring RNA quality control and regulating gene expression. Mutations in the DIS3 gene are strongly linked to multiple myeloma, a hematologic malignancy, where they correlate with disease progression and poor prognosis. These mutations often impair DIS3’s enzymatic functions, leading to dysregulated RNA homeostasis and genomic instability.
DIS3 antibodies are widely used in research to detect protein expression, localization, and functional alterations in both normal and pathological contexts. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping elucidate DIS3’s role in RNA processing and its association with cancer mechanisms. Studies using these antibodies have revealed reduced DIS3 expression in myeloma cells, suggesting its tumor-suppressive potential. Additionally, DIS3 antibodies aid in exploring interactions with other exosome subunits or RNA molecules, providing insights into RNA decay pathways. Their application continues to advance understanding of DIS3’s dual roles in maintaining cellular RNA integrity and contributing to oncogenesis when dysregulated.
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