| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2 |
| Entrez GeneID | 1787 |
| WB Predicted band size | 44.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human Dnmt2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3-4条与Dnmt2抗体相关的参考文献及摘要概括：
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1. **文献名称**：*Dnmt2 mediates targeted methylation of histidine tRNA and is required for embryonic development*
**作者**：Schaefer, M. et al.
**摘要**：该研究利用Dnmt2抗体通过免疫沉淀和Western blot分析，揭示了Dnmt2特异性催化tRNAHis的胞嘧啶甲基化，并证明其在小鼠胚胎发育中具有关键作用，缺失Dnmt2会导致胚胎致死。
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2. **文献名称**：*Conservation of DNA methylation in Drosophila melanogaster*
**作者**：Lyko, F. et al.
**摘要**：作者通过Dnmt2抗体的免疫荧光染色，发现果蝇中Dnmt2蛋白主要定位于细胞质，而非细胞核，支持了Dnmt2可能参与RNA而非DNA甲基化的假说，挑战了其传统分类为DNA甲基转移酶的观点。
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3. **文献名称**：*The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity*
**作者**：Goll, M.G. et al.
**摘要**：研究使用Dnmt2抗体进行蛋白质印迹和酶活性分析，证实人源Dnmt2在体外具有微弱的DNA甲基化活性，但其主要功能可能转向RNA修饰，尤其在应激反应中调控tRNA稳定性。
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4. **文献名称**：*Dnmt2 deficiency impacts cancer progression by regulating mTOR signaling*
**作者**：Tucci, V. et al.
**摘要**：通过Dnmt2抗体的免疫组化检测，发现Dnmt2在多种癌症组织中高表达，并通过调控mTOR通路影响肿瘤代谢和生长，为靶向Dnmt2的抗癌治疗提供了潜在依据。
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以上文献均涉及Dnmt2抗体的实验应用(如Western blot、免疫荧光等)，并围绕Dnmt2的酶活性、亚细胞定位及生物学功能展开研究。
The Dnmt2 antibody is a crucial tool for studying the Dnmt2 protein, a member of the DNA methyltransferase family. Initially classified as a DNA methyltransferase due to its conserved catalytic motifs, Dnmt2 (also known as tRNA aspartic acid methyltransferase or TRDMT1) was later shown to primarily methylate tRNA rather than DNA. This unique function distinguishes it from other Dnmt family members (Dnmt1. Dnmt3a/b), which catalyze DNA methylation at CpG sites. Dnmt2’s role in epigenetic regulation remains debated, though studies suggest it influences gene expression, stress responses, and developmental processes through tRNA methylation, impacting translation fidelity and small RNA processing.
Antibodies targeting Dnmt2 enable researchers to investigate its expression, localization, and interactions across tissues and model organisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Due to Dnmt2’s evolutionary conservation, these antibodies often exhibit cross-reactivity in diverse species, including humans, mice, and Drosophila. However, specificity validation is critical, as some commercial antibodies may cross-react with unrelated proteins or other Dnmt family members. Recent studies using Dnmt2-knockout models have helped verify antibody reliability. Research applications span cancer biology, neurodevelopment, and epigenetics, particularly in contexts linking tRNA modification to disease mechanisms. Despite its enigmatic role, Dnmt2 remains a focal point for exploring non-canonical methylation pathways, underscoring the importance of high-quality antibodies in elucidating its biological functions.
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