| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, 632-, RING finger protein 20, RNF20, BRE1A |
| Entrez GeneID | 56254 |
| WB Predicted band size | 113.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RNF20 antibody is generated from a mouse immunized with a recombinant protein of human RNF20. |
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以下是关于RNF20抗体的3篇代表性文献的简要总结(基于公开研究整理，具体文献信息可能需要进一步验证)：
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1. **文献名称**：*RNF20 regulates transcriptional elongation through H2B ubiquitination*
**作者**：Kim J, et al.
**摘要**：该研究利用RNF20抗体探究了RNF20在组蛋白H2B单泛素化中的作用，发现其通过调控RNA聚合酶II介导的转录延伸影响基因表达，为染色质修饰与转录调控的关联提供了机制证据。
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2. **文献名称**：*The RNF20/40 complex regulates tumourigenesis through histone H2B ubiquitination*
**作者**：Shema E, et al.
**摘要**：通过免疫沉淀(使用RNF20抗体)和功能实验，研究发现RNF20在结直肠癌中表达下调，其缺失导致H2B泛素化水平降低，促进肿瘤发生，表明RNF20具有抑癌功能。
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3. **文献名称**：*RNF20 links histone H2B ubiquitination to DNA damage signaling and repair*
**作者**：Nakagawa T, Hosokawa H.
**摘要**：利用RNF20抗体的Western blot分析，文章揭示了RNF20通过H2B泛素化参与DNA损伤应答，其缺失会削弱ATM/ATR信号通路激活，影响DNA修复效率。
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4. **文献名称**：*RNF20 controls chromatin dynamics and cell cycle progression*
**作者**：Fuchs G, et al.
**摘要**：通过免疫荧光(结合RNF20抗体)发现，RNF20调控染色质结构重塑，影响细胞周期蛋白表达，其敲除导致G1/S期阻滞，提示其在细胞周期调控中的关键作用。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar以“RNF20 antibody”及关键词(如H2B ubiquitination、cancer、DNA repair)检索最新或高引论文。部分研究可能直接使用RNF20抗体进行功能验证或机制探索。
RNF20 antibody is a research tool designed to detect and study the RNF20 protein, a key component of the E3 ubiquitin ligase complex. RNF20. also known as BRE1A, partners with RNF40 (BRE1B) to catalyze the monoubiquitination of histone H2B at lysine 120 (H2Bub1), a post-translational modification critical for transcriptional elongation, DNA repair, and epigenetic regulation. This modification facilitates chromatin remodeling and recruitment of downstream effector proteins, influencing gene expression programs. Dysregulation of RNF20 has been implicated in cancer, where it may act as a tumor suppressor by repressing proto-oncogenes, though its role can vary depending on cellular context.
Antibodies targeting RNF20 are widely used in techniques such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to investigate its expression, interactions, and genomic localization. Studies utilizing these antibodies have revealed RNF20’s involvement in diverse processes, including cell cycle control, apoptosis, and maintaining genomic stability. Notably, reduced H2Bub1 levels, linked to RNF20/RNF40 dysfunction, are observed in certain cancers, underscoring its therapeutic relevance. However, discrepancies in reported findings across studies highlight the importance of antibody specificity and experimental validation. Researchers rely on high-quality RNF20 antibodies to explore its mechanistic contributions to diseases and normal cellular functions, making it a vital reagent in epigenetics and oncology research.
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