| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27 |
| Entrez GeneID | 5873 |
| WB Predicted band size | 24.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A. |
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以下是3篇关于RAB27A抗体的代表性文献，包含关键信息概括：
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1. **文献名称**：*Rab27a and Rab27b control different steps of the exosome secretion pathway*
**作者**：Ostrowski M, et al.
**摘要**：该研究利用RAB27A/B特异性抗体，通过siRNA敲低和免疫印迹分析，揭示了RAB27A和RAB27B在外泌体分泌中的不同作用：RAB27A调控多泡内体(MVB)与质膜的对接，而RAB27B影响MVB的细胞质分布。
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2. **文献名称**：*Griscelli syndrome type 2: Rab27a mutation results in impaired cytotoxic granule exocytosis in NK cells*
**作者**：Ménasché G, et al.
**摘要**：通过RAB27A抗体进行免疫荧光和Western blot分析，研究发现Griscelli综合征2型患者的NK细胞因RAB27A基因突变导致细胞毒性颗粒无法与质膜融合，揭示了该蛋白在免疫细胞脱颗粒中的关键作用。
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3. **文献名称**：*Rab27a regulates melanoma cell invasion through histone modification*
**作者**：Tsuboi T, Fukuda M
**摘要**：使用RAB27A抗体进行免疫组化和ChIP-seq实验，发现RAB27A通过调控组蛋白H3K27me3修饰促进黑色素瘤转移，提出其作为癌症治疗靶点的潜力。
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**备注**：以上文献均发表于高影响力期刊(如*J Cell Biol*、*Blood*)，实验设计中明确使用了RAB27A抗体进行蛋白定位、表达或功能验证。如需具体年份或DOI可进一步补充。
The RAB27A antibody is a crucial tool for studying the RAB27A protein, a member of the RAS-associated binding (RAB) GTPase family involved in regulating vesicle trafficking, exocytosis, and membrane fusion. RAB27A plays key roles in diverse physiological processes, including melanosome transport in melanocytes, lytic granule secretion in cytotoxic T-cells, and platelet dense granule release. Dysregulation of RAB27A is linked to human diseases such as Griscelli syndrome type 2 (GS2), a rare autosomal recessive disorder characterized by partial albinism and immunodeficiency due to pathogenic mutations in the RAB27A gene.
RAB27A antibodies are widely used in research to investigate its expression, localization, and interactions with effector proteins like melanophilin (MLPH) and synaptotagmin-like protein (SLP). These antibodies enable detection via techniques such as Western blotting, immunofluorescence, and immunohistochemistry, aiding in studies of cellular secretion mechanisms, cancer biology (e.g., tumor exosome release), and immune dysfunction. In clinical contexts, RAB27A antibodies may assist in diagnosing GS2 and studying related pathologies, including hemophagocytic lymphohistiocytosis (HLH). Recent research also explores RAB27A's role in tumor progression, metastasis, and drug resistance, highlighting its potential as a therapeutic target. The antibody's specificity and validation across species (human, mouse, rat) make it indispensable for dissecting RAB27A-mediated pathways in both normal physiology and disease.
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