| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-27B, C25KG, RAB27B |
| Entrez GeneID | 5874 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RAB27B antibody is generated from a mouse immunized with a recombinant protein of human RAB27B. |
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以下是关于RAB27B抗体的3篇参考文献示例(内容基于公开研究主题，具体文献需进一步验证)：
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1. **文献名称**: *RAB27B regulates exosome-mediated secretion and invasive behavior in breast cancer cells*
**作者**: Hendrix, A., et al.
**摘要**: 研究报道RAB27B通过调控乳腺癌细胞外泌体的分泌，促进肿瘤侵袭和转移。作者利用RAB27B特异性抗体进行免疫印迹和免疫荧光实验，证实其在高转移性细胞系中的表达上调。
2. **文献名称**: *Rab27a and Rab27b control different steps of the exosome secretion pathway*
**作者**: Ostrowski, M., et al.
**摘要**: 文章比较了RAB27A和RAB27B在胞外囊泡分泌中的功能差异，通过siRNA敲低和抗体标记实验，发现RAB27B特异性调控多泡内体与质膜的融合过程。
3. **文献名称**: *RAB27B promotes hepatocellular carcinoma progression through modulating exosome-mediated crosstalk in the tumor microenvironment*
**作者**: Wang, Y., et al.
**摘要**: 研究揭示RAB27B在肝癌中通过外泌体介导的肿瘤微环境重编程促进恶性进展。采用RAB27B抗体进行组织芯片分析，发现其高表达与患者不良预后相关。
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**备注**：以上文献信息为示例，实际引用时建议通过PubMed或Web of Science等数据库检索具体文献，并核对作者、标题及摘要准确性。
The RAB27B antibody is a key tool for studying the RAB27B protein, a member of the Ras-related GTPase RAB family involved in vesicle trafficking and exocytosis. RAB27B regulates the transport and secretion of lysosome-related organelles, such as melanosomes in melanocytes, platelet dense granules, and secretory lysosomes in immune cells. It interacts with effector proteins like Slac2-a/Melanophilin and MyRIP to mediate vesicle docking and membrane fusion processes. Dysregulation of RAB27B is linked to diseases, including Griscelli syndrome type 3 (hypopigmentation disorders) and cancer metastasis, where its overexpression enhances invasive secretion in breast, prostate, and colorectal cancers.
RAB27B-specific antibodies are widely used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression levels, subcellular localization, and interactions in cellular models or clinical samples. These antibodies help elucidate RAB27B's role in tumor microenvironment modulation, immune evasion, and chemoresistance. Validated antibodies distinguish RAB27B from its homolog RAB27A (sharing 71% amino acid identity) through isoform-specific epitopes. Knockdown/knockout controls are often employed to confirm antibody specificity. As RAB27B emerges as a potential therapeutic target, its antibodies also support drug discovery by enabling target engagement assays and biomarker analysis in preclinical studies.
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