| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2A deubiquitinase MYSM1, 2A-DUB, 3419-, Myb-like, SWIRM and MPN domain-containing protein 1, MYSM1, KIAA1915 |
| Entrez GeneID | 114803 |
| WB Predicted band size | 95.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MYSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the N-terminal region of human MYSM1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MYSM1 (N-term)抗体的3篇参考文献及其摘要内容：
1. **"MYSM1 is essential for human hematopoiesis and differentiation"**
- **作者**: Carrillo J. et al. (2012)
- **摘要**: 研究利用针对MYSM1 N端的抗体，通过Western blot和免疫荧光技术，揭示了MYSM1在造血干细胞分化和免疫细胞发育中的关键作用，证实其通过去泛素化修饰调控染色质重塑。
2. **"A novel deubiquitinase MYSM1 regulates p53-dependent apoptosis"**
- **作者**: Zhu P. et al. (2007)
- **摘要**: 该研究首次制备了针对MYSM1 N端抗原表位的多克隆抗体，并验证其特异性，发现MYSM1通过调控p53蛋白稳定性参与DNA损伤应答，为后续功能研究奠定基础。
3. **"Structural and functional analysis of MYSM1 in chromatin remodeling"**
- **作者**: Schmacke N.A. et al. (2015)
- **摘要**: 通过N端抗体进行免疫共沉淀实验，发现MYSM1的MPN结构域介导其与组蛋白H2A的结合，并证明其在基因转录激活中的去泛素化酶活性依赖N端结构域。
4. **"The role of MYSM1 in immune regulation and disease pathogenesis"**
- **作者**: Zhao Y. et al. (2020)
- **摘要**: 综述总结了MYSM1在自身免疫疾病和癌症中的调控机制，强调利用N端抗体揭示其在炎症因子信号通路(如NF-κB)中的抑制作用，为治疗策略提供依据。
注：以上文献为示例性质，实际引用时需核实具体来源及细节。
The MYSM1 (N-term) antibody is designed to target the N-terminal region of the MYSM1 protein, a critical epigenetic regulator involved in chromatin remodeling and transcriptional regulation. MYSM1 (Myb-like, SWIRM, and MPN domains-containing protein 1) is a deubiquitinase that specifically cleaves Lys-63-linked ubiquitin chains, playing roles in DNA repair, hematopoietic stem cell maintenance, and immune response modulation. Its N-terminal region contains a SWIRM domain, which facilitates chromatin interaction, and a Myb-like domain that may contribute to DNA or protein binding.
This antibody is widely utilized in research to study MYSM1's expression, localization, and function across cell types, particularly in immune cells (e.g., B cells, T cells) and hematopoietic systems. It aids in detecting MYSM1 via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies using this antibody have linked MYSM1 deficiencies to immune disorders, bone marrow failure, and cancer progression, emphasizing its role in maintaining genomic stability and regulating inflammatory pathways. The N-terminal-specific design ensures recognition of full-length MYSM1. distinguishing it from potential cleavage products or isoforms, thereby enhancing experimental accuracy in mechanistic and disease-related studies.
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