关键信息
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基因名
rpsK
- 应用
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别名
rpsK；Small ribosomal subunit Protein uS11
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7R9
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表达区间
2-129aa
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氨基酸序列
AKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV
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分子量
17.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RpsK is a crucial ribosomal protein that plays a significant role in the assembly and function of the ribosomal subunit in bacteria. It is part of the 30S ribosomal subunit and is essential for protein synthesis, influencing the fidelity of translation and stability of the ribosome. The study of RpsK, particularly as a recombinant protein, has garnered attention due to its potential implications in understanding bacterial physiology and the mechanisms of antibiotic resistance. Recombinant RpsK can be produced through genetic engineering techniques, allowing for detailed structural and functional analyses. This research is critical in unraveling the intricate processes of bacterial translation, which can lead to the identification of new drug targets. Furthermore, with the rise of multi-drug resistant bacterial strains, a deeper understanding of RpsK could offer insights that contribute to the development of novel antibiotics or therapeutic strategies. By characterizing RpsK and its interactions within the ribosomal assembly, researchers aim to bridge gaps in knowledge regarding ribosomal function and its regulation, ultimately aiding the fight against bacterial infections.
