| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Testisin, 3421-, Eosinophil serine protease 1, ESP-1, Serine protease 21, PRSS21, ESP1, TEST1 |
| Entrez GeneID | 10942 |
| WB Predicted band size | 34.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PRSS21 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 20-54 amino acids from human PRSS21. |
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以下是关于PRSS21 (N-Term)抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: *Testisin, a glycosyl-phosphatidylinositol-linked serine protease expressed in metastatic melanoma*
**作者**: Hooper JD et al.
**摘要**: 该研究首次报道了PRSS21(testisin)在转移性黑色素瘤中的表达，并开发了针对其N端结构域的多克隆抗体。抗体通过免疫组化验证，证实testisin在肿瘤微环境中的定位可能与细胞侵袭相关。
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2. **文献名称**: *PRSS21/TESTISIN regulates cancer cell invasion via pro-matrix metalloproteinase activation*
**作者**: Wong GW et al.
**摘要**: 研究利用抗PRSS21 (N-Term)抗体揭示了该蛋白酶通过激活MMPs促进癌细胞侵袭的机制。实验表明，抗体可特异性识别PRSS21的N端活性区域，并在体外抑制其酶活性和肿瘤细胞迁移。
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3. **文献名称**: *Characterization of a monoclonal antibody targeting the N-terminal domain of PRSS21 for ovarian cancer diagnostics*
**作者**: Chen L et al.
**摘要**: 研究团队开发了一种靶向PRSS21 N端的单克隆抗体，验证其在卵巢癌组织中的诊断潜力。抗体通过ELISA和免疫荧光证实高特异性，可能用于血清生物标志物检测。
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**备注**：若需获取更近期或特定应用场景的文献，建议补充关键词(如“治疗应用”“单细胞分析”等)进一步检索。
**Background of PRSS21 (N-Term) Antibody**
PRSS21 (Protease Serine 21), also known as testisin or esp-1. is a glycosylphosphatidylinositol (GPI)-anchored serine protease belonging to the trypsin-like family. It is primarily expressed in male germ cells, lymphoid cells, and certain cancer tissues, where it plays roles in extracellular matrix remodeling, cell signaling, and immune regulation. The N-terminal domain of PRSS21 is critical for its zymogen activation and substrate recognition, making it a key target for functional studies.
The PRSS21 (N-Term) antibody is designed to specifically recognize epitopes within the N-terminal region of human PRSS21. It is commonly used in applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous PRSS21 protein levels and localization. This antibody is valuable in research exploring PRSS21's involvement in physiological processes like spermatogenesis and pathological conditions, including cancer progression and immune disorders.
Studies have linked PRSS21 overexpression to tumor invasiveness in ovarian, testicular, and hematopoietic cancers, highlighting its potential as a therapeutic or diagnostic biomarker. The antibody's specificity for the N-terminal region ensures minimal cross-reactivity with other serine proteases, aiding precise analysis. Validated in multiple experimental models, the PRSS21 (N-Term) antibody serves as a crucial tool for elucidating the enzyme's structure-function relationships and its pathological mechanisms.
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