| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | POU domain, class 5, transcription factor 1, POU domain protein 2, pou5f1, gp-9, pou-2, pou2 |
| Entrez GeneID | 30333 |
| WB Predicted band size | 51.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This Zebrafish pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-132 amino acids from the Central region of Zebrafish pou5f1. |
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以下是关于斑马鱼Pou5f1抗体的3篇参考文献的简要总结：
1. **文献名称**：*"Characterization of zebrafish Pou5f1 protein expression using a novel monoclonal antibody"*
**作者**：Takeda H, et al.
**摘要**：该研究开发了一种特异性识别斑马鱼Pou5f1的单克隆抗体，通过免疫组织化学和Western blot验证其在早期胚胎中的表达模式，发现Pou5f1在囊胚期广泛分布，并在神经板形成阶段逐渐局限于前脑区域。
2. **文献名称**：*"Functional analysis of zebrafish pou5f1 in neural crest development"*
**作者**：Burg L, et al.
**摘要**：利用Pou5f1抗体结合免疫荧光技术，作者揭示了该转录因子在神经嵴细胞分化中的关键作用。抗体染色显示，Pou5f1缺失导致神经嵴标志物Sox10表达显著下降，提示其通过调控下游基因参与发育调控。
3. **文献名称**：*"Dynamic expression of Pou5f1 during zebrafish gastrulation revealed by antibody-based imaging"*
**作者**：Reim G, et al.
**摘要**：通过高分辨率共聚焦显微镜结合Pou5f1抗体，研究追踪了该蛋白在体轴形成过程中的动态分布，发现其梯度表达与中胚层诱导密切相关，抗体特异性通过CRISPR基因敲除实验验证。
4. **文献名称**：*"Pou5f1 cooperates with SoxB1 factors in zebrafish endoderm formation"*
**作者**：Xu Y, et al.
**摘要**：该研究使用Pou5f1抗体进行双荧光染色，证明其与SoxB1因子在内胚层前体细胞中共定位，并通过功能实验揭示二者协同调控下游靶基因Gata5的表达，推动内胚层分化。
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**注**：上述文献为示例性质，实际引用时需根据具体研究内容选择。若需真实文献，建议检索PubMed或Web of Science并筛选抗体应用相关研究。
The zebrafish pou5f1 antibody is a critical tool for studying early embryonic development and pluripotency in this model organism. Pou5f1. a member of the POU-domain transcription factor family, is functionally homologous to mammalian Oct4 and plays essential roles in maintaining pluripotency of embryonic stem cells. In zebrafish, the pou5f1 gene (also called oct4 or pou2) is maternally deposited and expressed during oogenesis, with zygotic transcription persisting through gastrulation. It regulates cell fate determination, germ layer formation, and neural development.
This antibody typically detects the zebrafish Pou5f1 protein across developmental stages, enabling visualization of its dynamic expression patterns via techniques like whole-mount immunofluorescence, immunohistochemistry, and Western blotting. It has been particularly valuable in characterizing pou5f1 mutants (e.g., spiel-ohne-grenzen mutants) and studying maternal-zygotic gene functions. Commercial and custom-generated versions are available, often raised against conserved epitopes in the N-terminal domain. Validation generally includes knockout controls and cross-reactivity checks with other POU family members (e.g., Pou5f3). Researchers frequently employ this reagent to investigate stem cell biology, germline development, and regeneration mechanisms in zebrafish, leveraging its conserved functional parallels with mammalian pluripotency networks. Key studies using this antibody have clarified evolutionary aspects of pluripotency regulation across vertebrates.
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