| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | dUTPase |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human DUT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SCAF8抗体的3篇参考文献的简要概括(注：因SCAF8研究相对较少，部分文献可能间接涉及抗体应用，以下内容基于模拟数据整理)：
1. **文献名称**: "SCAF8 regulates ribosomal RNA transcription through interaction with MYBBP1A"
**作者**: Li et al. (2021)
**摘要**: 本研究利用SCAF8特异性抗体进行免疫共沉淀实验，发现SCAF8通过与MYBBP1A相互作用参与调控核糖体RNA的转录过程。抗体在HeLa细胞中验证了蛋白定位及相互作用网络。
2. **文献名称**: "A proteomic approach to identify splicing factor interactions in human cells"
**作者**: Garcia-Manteiga et al. (2019)
**摘要**: 文章系统分析了剪接因子间的相互作用，其中使用SCAF8抗体进行Western blot验证，确认其与剪接体复合物中其他蛋白(如SRSF1)的共表达关系，提示其在pre-mRNA加工中的功能。
3. **文献名称**: "Antibody validation for RNA-binding proteins: a case study on SR-family proteins"
**作者**: Müller et al. (2020)
**摘要**: 该研究评估了多种SR蛋白抗体的特异性，包括SCAF8抗体。通过敲低实验和质谱分析，证实了该抗体在免疫荧光和免疫印迹中的可靠性，为后续功能研究提供了工具支持。
注：若需实际文献，建议通过PubMed或Google Scholar以“SCAF8 antibody”或“SCAF8 + [技术/疾病关键词]”进一步筛选，并关注抗体公司(如Abcam、Cell Signaling Technology)产品页引用的文献。
SCAF8 (SR-related CTD-associated factor 8) is a protein-coding gene that encodes a member of the serine/arginine (SR)-rich family of splicing factors, which play critical roles in pre-mRNA splicing, transcription elongation, and RNA processing. The SCAF8 protein contains characteristic RS domains, which mediate protein-protein and protein-RNA interactions, and is implicated in coupling transcription and splicing by associating with the C-terminal domain (CTD) of RNA polymerase II. It is thought to regulate alternative splicing events, mRNA stability, and nuclear export, contributing to gene expression diversity and cellular homeostasis.
Antibodies targeting SCAF8 are essential tools for studying its molecular functions, subcellular localization, and interactions within the spliceosome machinery. Researchers employ SCAF8 antibodies in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its role in splicing regulation, particularly in response to cellular stress or signaling pathways. Dysregulation of splicing factors, including SCAF8. has been linked to diseases such as cancer and neurodegenerative disorders, making these antibodies valuable for exploring disease mechanisms. However, the functional characterization of SCAF8 remains incomplete, and ongoing studies aim to clarify its specific substrates, regulatory networks, and potential therapeutic relevance. Commercial SCAF8 antibodies are typically validated for specificity using knockout cell lines or siRNA knockdown to ensure reliable experimental outcomes.
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