Rabbit Polyclonal (DANRE)opn4l Antibody

The DANRE (Danio rerio) Opn4l antibody is a specialized tool used to detect Opn4l, a long-wavelength-sensitive opsin protein in zebrafish. Opn4l belongs to the melanopsin family of photoreceptive proteins, primarily involved in non-image-forming visual processes such as circadian rhythm regulation and light-dependent behavior. Zebrafish, a model organism in developmental and neurobiological research, express multiple opsin variants, with Opn4l playing a role in retinal and extra-retinal light sensing. This antibody is typically raised in hosts like rabbits or mice using peptide antigens derived from conserved regions of the Opn4l protein. It enables researchers to localize and quantify Opn4l expression via techniques like immunohistochemistry, Western blotting, or immunofluorescence. Validations often include knockout controls or peptide-blocking assays to confirm specificity. Opn4l's study contributes to understanding light-mediated processes in vertebrates, including circadian entrainment, larval phototaxis, and seasonal behavior modulation. The antibody’s utility extends to comparative studies of vertebrate photoreception evolution and screening genetic models with disrupted light-signaling pathways. Proper storage and standardized protocols ensure reproducibility in experimental outcomes.