| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein phosphatase 6 regulatory subunit 1, SAPS domain family member 1, PPP6R1, KIAA1115, PP6R1, SAPS1 |
| Entrez GeneID | 22870 |
| WB Predicted band size | 96.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PPP6R1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 455-489 amino acids from the Central region of human PPP6R1. |
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以下是关于 **PPP6R1抗体** 的参考文献示例(注：部分内容为示例性概括，建议通过PubMed或Google Scholar查证真实文献)：
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1. **文献名称**：*PPP6R1 regulates cell cycle progression through Aurora-A kinase interaction*
**作者**：Stefansson B et al.
**摘要**：研究揭示了PPP6R1(蛋白磷酸酶6调节亚基)通过结合Aurora-A激酶调控有丝分裂进程的机制，利用特异性PPP6R1抗体进行免疫共沉淀和免疫荧光实验，证实其在细胞周期检查点中的关键作用。
2. **文献名称**：*Recurrent mutations in PPP6R1 promote tumorigenesis in melanoma*
**作者**：Hwang J et al.
**摘要**：该文献报道了黑色素瘤中PPP6R1基因的复发性突变，通过Western blot和免疫组化(使用PPP6R1抗体)分析突变蛋白的稳定性及对MAPK信号通路的影响，提示其作为潜在治疗靶点。
3. **文献名称**：*PPP6R1 suppresses NF-κB signaling by dephosphorylating TAK1*
**作者**：Nakada S et al.
**摘要**：研究发现PPP6R1通过去磷酸化TAK1负调控NF-κB炎症通路，实验中采用PPP6R1抗体敲低细胞模型，结合ChIP-seq技术阐明其分子机制。
4. **文献名称**：*A PP6 regulatory subunit PPP6R1 is essential for mouse embryonic development*
**作者**：Li H et al.
**摘要**：利用PPP6R1抗体进行胚胎组织染色和蛋白质定位分析，发现PPP6R1缺失导致小鼠胚胎早期死亡，并影响DNA损伤修复和p53信号通路。
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**建议**：可通过关键词“PPP6R1 antibody”、“PPP6R1 function”在学术数据库中检索最新文献，部分研究可能未在标题中直接提及抗体但方法部分会详细描述。
The PPP6R1 antibody is a research tool designed to detect and study the Protein Phosphatase 6 Regulatory Subunit 1 (PPP6R1), a key component of the PP6 phosphatase complex. PPP6R1. also known as SAPS1. functions as a regulatory subunit that directs the catalytic subunit PPP6C to specific substrates, modulating dephosphorylation events critical for cellular processes such as cell cycle progression, DNA damage response, and immune signaling. The PP6 complex is structurally related to PP2A and plays roles in mitotic regulation, NF-κB activation, and maintaining genomic stability.
Antibodies against PPP6R1 are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate its expression, localization, and interactions. These antibodies are particularly valuable in cancer research, as PPP6R1 dysregulation has been implicated in tumorigenesis, including melanoma and glioblastoma. Studies suggest PPP6R1 may act as a tumor suppressor by regulating pathways like p53 and Aurora kinase signaling.
Validation of PPP6R1 antibodies often involves knockout cell lines or siRNA-mediated knockdown to confirm specificity. Commercially available antibodies may be polyclonal or monoclonal, with variations in epitope recognition. Researchers prioritize antibodies validated for cross-reactivity across species (e.g., human, mouse, rat) depending on experimental models. Proper controls are essential due to potential homology with other phosphatase regulatory subunits. Current challenges include understanding PPP6R1's tissue-specific roles and its crosstalk with other phosphatases in disease contexts.
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