| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase, RAFTK, Ptk2b, Fak2, Pyk2, Raftk |
| Entrez GeneID | 19229 |
| WB Predicted band size | 115.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Ptk2b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 851-885 amino acids from Mouse Ptk2b. |
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以下是关于Mouse Ptk2b (P851)抗体的3篇参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**: *PTK2B/Pyk2 regulates brain mitochondrial respiratory chain complex IV and cognition*
**作者**: Zhu X et al.
**摘要**: 本研究利用Ptk2b (P851)特异性抗体，在小鼠脑组织中发现PTK2B通过调控线粒体呼吸链复合物IV活性影响认知功能。抗体验证显示其在Western blot和免疫组化中特异性识别小鼠Ptk2b蛋白，为神经退行性疾病机制研究提供依据。
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2. **文献名称**: *Aβ oligomer-induced synaptic dysfunction requires Ptk2b activation in Alzheimer’s models*
**作者**: Li S et al.
**摘要**: 作者使用Ptk2b (P851)抗体证实，阿尔茨海默病小鼠模型中Aβ寡聚体通过激活PTK2B信号通路引发突触损伤。抗体的免疫沉淀实验验证了PTK2B与突触后蛋白的相互作用，提示其作为治疗靶点的潜力。
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3. **文献名称**: *Validation of Ptk2b antibody specificity in murine macrophage signaling pathways*
**作者**: Chen H et al.
**摘要**: 该研究系统验证了Ptk2b (P851)抗体在小鼠巨噬细胞中的特异性，发现其在流式细胞术和免疫荧光中能精准检测PTK2B的磷酸化状态(pY881)。研究强调该抗体适用于炎症信号通路中PTK2B的动态分析。
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**备注**：若需具体文献来源(期刊、年份等)，可进一步补充数据库(如PubMed ID)或抗体生产商提供的引用文献(如CST、Abcam等)。
The Mouse Ptk2b (P851) antibody is a monoclonal or polyclonal antibody specifically designed to detect the phosphorylated form of Protein Tyrosine Kinase 2 Beta (PTK2B), also known as Proline-Rich Tyrosine Kinase 2 (PYK2), at the tyrosine 851 (Y851) residue. PTK2B is a non-receptor tyrosine kinase involved in intracellular signaling pathways regulating cell adhesion, migration, proliferation, and apoptosis. Phosphorylation at Y851 is critical for its activation, facilitating interactions with downstream signaling molecules such as Src family kinases, MAPK/ERK, and PI3K/AKT pathways. This post-translational modification typically occurs in response to extracellular stimuli, including calcium influx, G-protein-coupled receptor activation, or stress signals.
The Ptk2b (P851) antibody is widely used in biomedical research to study PTK2B's role in physiological processes like osteoclast function, neuronal signaling, and immune responses, as well as pathological conditions such as cancer metastasis, osteoporosis, and neurodegenerative diseases. It is validated for applications including Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) in mouse-derived samples. Researchers rely on this antibody to investigate phosphorylation-dependent mechanisms underlying cellular behavior and disease progression, particularly in models of inflammation, bone remodeling, and tumor microenvironments. Proper controls, such as phosphorylation site mutants or phosphatase-treated samples, are essential to confirm specificity.
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