Rabbit Polyclonal (Mouse)Notch1 Antibody

The Notch1 receptor is a critical component of the Notch signaling pathway, an evolutionarily conserved system regulating cell differentiation, proliferation, and apoptosis. As a transmembrane protein, Notch1 undergoes proteolytic cleavage upon ligand binding, releasing its intracellular domain (NICD) to initiate transcriptional regulation of target genes. Dysregulation of Notch1 is implicated in cancers, cardiovascular disorders, and developmental defects. Mouse Notch1 antibodies are essential tools for detecting and studying this receptor in murine models, which are widely used to mimic human diseases. These antibodies typically target specific epitopes, such as extracellular domains involved in ligand interaction or intracellular regions critical for signaling. They enable techniques like Western blotting, immunohistochemistry, flow cytometry, and immunofluorescence to assess Notch1 expression, localization, and activation status. Validation often includes knockout controls to confirm specificity. Research applications span cancer biology (e.g., studying tumor angiogenesis or leukemia), stem cell maintenance, and tissue development. Commercially available Mouse Notch1 antibodies vary in clonal origin (monoclonal/polyclonal), host species, and conjugation formats, allowing flexibility in experimental design. Proper selection depends on the target epitope, sample type, and intended method. Recent studies also utilize these antibodies to explore Notch1's role in immune modulation and its potential as a therapeutic target.