| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | F-box/WD repeat-containing protein 7, Archipelago homolog, hAgo, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4, FBXW7 (HGNC:16712) |
| Entrez GeneID | 55294 |
| WB Predicted band size | 79.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This FBXW7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 177-208 amino acids from the N-terminal region of human FBXW7. |
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以下是关于FBXW7 (N-term)抗体的3篇参考文献的简要信息：
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1. **标题**：*FBXW7 regulates Notch signaling in hematopoietic stem cell homeostasis*
**作者**：Thompson BJ et al.
**摘要**：该研究通过免疫沉淀和Western blot技术，使用FBXW7 N端抗体，揭示了FBXW7在造血干细胞中通过靶向降解Notch受体维持稳态的机制，证实其缺失导致白血病发生风险增加。
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2. **标题**：*FBXW7 mutations impair substrate degradation and promote colorectal tumorigenesis*
**作者**：Davis H et al.
**摘要**：研究分析了FBXW7在结直肠癌中的突变谱，通过N端抗体检测肿瘤组织中FBXW7蛋白表达水平，发现突变体丧失对c-Myc等底物的泛素化能力，促进肿瘤进展。
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3. **标题**：*SCF-FBXW7 regulates cell cycle by targeting cyclin E for ubiquitination*
**作者**：Koepp DM et al.
**摘要**：该研究利用FBXW7 N端抗体进行免疫荧光和Western blot，证明FBXW7通过调控细胞周期蛋白Cyclin E的降解，影响G1/S期转换，其功能缺失导致基因组不稳定。
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这些文献均通过FBXW7 N端抗体探究其在肿瘤发生、信号通路及细胞周期中的功能，实验方法涵盖Western blot、免疫沉淀及免疫荧光技术。如需具体DOI或发表年份，可进一步补充检索。
The FBXW7 (N-term) antibody is designed to target the N-terminal region of the F-box/WD repeat-containing protein 7 (FBXW7), a critical component of the ubiquitin-proteasome system. FBXW7 functions as a substrate recognition subunit within the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex, facilitating the degradation of key regulatory proteins involved in cell cycle progression, differentiation, and survival. Notable substrates include oncoproteins such as c-Myc, Cyclin E, and Notch, underscoring FBXW7's role as a tumor suppressor. Dysregulation or mutations in FBXW7 are linked to various cancers, including colorectal, breast, and hematological malignancies.
The N-terminal-specific antibody recognizes epitopes within the variable N-terminal domain of FBXW7. which is essential for substrate binding and interaction with other components of the SCF complex. This antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to detect FBXW7 expression levels, assess post-translational modifications, or investigate its subcellular localization. Researchers also employ it to study FBXW7 isoform-specific functions (α, β, γ), which arise from alternative splicing and differ in subcellular distribution. Given FBXW7's tumor-suppressive role, the antibody serves as a vital tool in cancer research, particularly for analyzing mutations or expression changes in clinical samples. Its specificity for the N-terminal region ensures minimal cross-reactivity with other F-box proteins, enhancing reliability in experimental models.
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