| WB | 1/500-1/4000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Nucleostemin, Gnl3, Ns |
| Entrez GeneID | 30877 |
| WB Predicted band size | 60.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Gnl3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 420-454 amino acids from the C-terminal region of Mouse Gnl3. |
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以下是关于小鼠Gnl3抗体的部分参考文献(示例为模拟生成内容，实际文献请查询数据库)：
1. **Smith et al. (2018)**
*标题*: "Characterization of Gnl3 Antibody in Mouse Embryonic Stem Cells"
*摘要*: 研究验证了Gnl3抗体在小鼠胚胎干细胞中的特异性，证实其在Western blot和免疫荧光中可检测Gnl3蛋白表达，并发现Gnl3在维持干细胞增殖中起关键作用。
2. **Chen et al. (2020)**
*标题*: "Gnl3 Expression Dynamics in Mouse Tumor Models"
*摘要*: 通过新型Gnl3抗体分析小鼠肿瘤模型中Gnl3的时空表达模式，发现其高表达与肿瘤干细胞活性及化疗耐药性相关。
3. **Johnson & Lee (2021)**
*标题*: "Development of a Monoclonal Antibody Against Mouse Gnl3 for In Vivo Studies"
*摘要*: 报道了一种高特异性小鼠Gnl3单克隆抗体的开发，成功用于流式细胞术和免疫组化，揭示Gnl3在再生肝脏组织中的动态表达。
4. **Review: Lee et al. (2022)**
*标题*: "Gnl3 Antibody Applications in Stem Cell and Cancer Research"
*摘要*: 综述了Gnl3抗体在小鼠模型中的技术应用进展，包括其在干细胞自我更新和癌症靶向治疗研究中的关键作用。
**注**：以上为示例性内容，实际文献需通过PubMed、Google Scholar等平台以“Mouse Gnl3 antibody”“Nucleostemin antibody”等关键词检索获取。
The mouse Gnl3 (Guanine nucleotide-binding protein-like 3), also known as Nucleostemin (NS), is a nucleolar GTPase implicated in cell proliferation, stem cell maintenance, and cancer biology. It is highly expressed in embryonic stem cells, certain adult stem cells, and rapidly dividing cancer cells, but downregulated in differentiated cells. Gnl3 plays a role in regulating ribosome biogenesis, cell cycle progression (particularly G1/S transition), and stress response pathways, including interactions with the p53 tumor suppressor network. Its dynamic nucleolar localization and GTP-dependent conformational changes are critical for its function in balancing cell proliferation and differentiation.
Mouse Gnl3 antibodies are essential tools for studying its expression, localization, and molecular interactions in murine models. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate Gnl3's roles in development, tissue regeneration, and oncogenesis. Specificity validation (e.g., using Gnl3-knockout controls) is crucial due to potential cross-reactivity with homologous proteins. Research applications span developmental biology (e.g., neural or hematopoietic stem cell studies), cancer research (tumor proliferation markers), and regenerative medicine, where Gnl3's modulation shows potential in reprogramming cell states. Recent studies also explore its involvement in aging-related cellular decline and chemotherapy resistance mechanisms.
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