| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-B4, Homeobox protein Hox-26, Hoxb4, Hox-26, Hoxb-4 |
| Entrez GeneID | 15412 |
| WB Predicted band size | 27.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Hoxb4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 218-252 amino acids from the C-terminal region of mouse Hoxb4. |
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以下是关于Mouse Hoxb4抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**：*Hoxb4 is required for hematopoietic stem cell expansion*
**作者**：Sauvageau, G. et al.
**摘要**：该研究通过Western blot和免疫组化技术，使用特异性Hoxb4抗体，证明Hoxb4在小鼠造血干细胞中的表达对其体外扩增至关重要，并揭示了其在造血发育中的调控机制。
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2. **文献名称**：*Overexpression of Hoxb4 enhances hematopoietic differentiation of embryonic stem cells*
**作者**：Antonchuk, J. et al.
**摘要**：研究利用Hoxb4抗体通过流式细胞术和免疫荧光技术，验证了Hoxb4过表达可促进小鼠胚胎干细胞向造血祖细胞分化，并加速体内外血细胞生成过程。
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3. **文献名称**：*Hoxb4 regulates proliferation and differentiation of hematopoietic stem cells through distinct mechanisms*
**作者**：Björnsson, J.M. et al.
**摘要**：该文献通过免疫沉淀和染色质免疫共沉淀(ChIP)结合Hoxb4抗体，阐明了Hoxb4在小鼠造血干细胞中通过调控靶基因(如Cyclin D1)平衡增殖与分化的分子机制。
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这些文献均使用了针对小鼠Hoxb4的特异性抗体，并在不同实验模型中揭示了其在干细胞生物学中的功能。如需更多文献或具体细节，建议通过PubMed或Web of Science进一步检索。
The mouse Hoxb4 antibody is a crucial tool in developmental biology and stem cell research, targeting the Hoxb4 protein encoded by the homeobox B4 gene. Hoxb4 belongs to the Hox gene family, which plays a pivotal role in regulating embryonic development, axial patterning, and cellular differentiation. Specifically, Hoxb4 is implicated in hematopoietic stem cell (HSC) self-renewal, neural development, and anterior-posterior body axis specification. Its overexpression has been shown to enhance HSC expansion while maintaining pluripotency, making it a focus in studies on blood cell regeneration and stem cell therapies.
This antibody is widely used to detect and quantify Hoxb4 expression in murine tissues and cell lines through techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Researchers employ it to investigate Hoxb4's regulatory mechanisms in normal development and diseases, such as leukemia, where dysregulated Hox genes are often observed. The antibody’s specificity is validated using knockout controls or peptide-blocking assays to ensure minimal cross-reactivity with other Hox proteins.
Typically produced in rabbits or mice, the antibody may be monoclonal or polyclonal, depending on the application. Its utility extends to both basic research and translational studies, aiding in uncovering Hoxb4's role in gene networks and potential therapeutic targets. Quality control involves testing for sensitivity, batch consistency, and performance in relevant experimental models.
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