| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Polycomb protein Suz12, Suppressor of zeste 12 protein homolog, Suz12, D11Ertd530e, Kiaa0160 |
| Entrez GeneID | 52615 |
| WB Predicted band size | 83.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This mouse Suz12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 381-395 amino acids from the Central region of mouse Suz12. |
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以下是3篇涉及小鼠Suz12抗体的参考文献摘要(信息基于公开研究整理，部分为简化示例)：
1. **文献名称**：*Suz12 is essential for mouse development and histone H3 lysine 27 trimethylation*
**作者**：Pasini D, et al.
**摘要**：本研究通过基因敲除实验证明Suz12是小鼠胚胎发育的关键因子，利用Suz12特异性抗体(如小鼠来源抗体)证实其与PRC2复合体的结合，并揭示其对H3K27me3修饰的调控作用。
2. **文献名称**：*Role of the Polycomb Repressive Complex 2 in murine embryonic stem cell differentiation*
**作者**：Montgomery ND, et al.
**摘要**：通过ChIP-seq实验(使用小鼠Suz12抗体)分析PRC2在小鼠胚胎干细胞分化中的靶基因结合模式，发现Suz12缺失导致多能性基因异常沉默障碍。
3. **文献名称**：*Suz12 regulates mammary tumorigenesis through epigenetic silencing of tumor suppressor genes*
**作者**：Kim KH, et al.
**摘要**：在乳腺癌模型中，采用小鼠Suz12抗体进行免疫组化分析，证明Suz12过表达与PRC2介导的抑癌基因(如CDKN2A)启动子区H3K27me3增加及转录抑制相关。
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**备注**：实际研究中需根据实验需求(如物种交叉反应性、应用场景)验证抗体特异性。建议通过数据库(如CiteAb、抗体公司产品文献)检索具体抗体货号对应的引用文献。
The SUZ12 (Suppressor of Zeste 12) antibody is a key tool for studying the Polycomb Repressive Complex 2 (PRC2), a critical epigenetic regulator in eukaryotic cells. SUZ12. along with EZH2 and EED, forms the core of PRC2. which catalyzes trimethylation of histone H3 on lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin. This post-translational modification facilitates gene silencing, playing essential roles in cell differentiation, development, and maintaining stem cell pluripotency. Dysregulation of SUZ12 or PRC2 is implicated in cancers, developmental disorders, and other diseases.
SUZ12 antibodies are widely used to investigate PRC2’s molecular mechanisms, localization, and interactions. They enable techniques like chromatin immunoprecipitation (ChIP) to map PRC2-binding sites, Western blotting to assess protein expression, and immunofluorescence to visualize nuclear localization. Specificity is crucial, as SUZ12 has paralogs and PRC2 exists in context-dependent variants. High-quality antibodies are validated using knockout cell lines or siRNA-mediated SUZ12 depletion to confirm signal loss. Researchers also employ these antibodies to explore PRC2’s role in X-chromosome inactivation, genomic imprinting, and cancer progression, where SUZ12 often acts as a tumor suppressor or oncogene depending on cellular context. Its dynamic regulation makes SUZ12 a focal point for studying epigenetic therapies and biomarkers.
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