| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD family member 1, SMAD 1, Smad1, Smad1, Madh1, Madr1 |
| Entrez GeneID | 17125 |
| WB Predicted band size | 52.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Smad1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-187 amino acids from the Central region of Mouse Smad1. |
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以下是关于Mouse Smad1抗体的3篇参考文献示例(内容为模拟概括，建议通过数据库核实具体文献)：
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1. **文献名称**: *"BMP-Smad1 signaling in embryonic development"*
**作者**: Massagué J, Chen YG.
**摘要**: 本研究利用小鼠模型和Smad1特异性抗体，通过Western blot和免疫组化分析，揭示了Smad1在骨形态发生蛋白(BMP)信号通路中的核心作用，尤其在胚胎肢体发育和细胞分化中的时空表达模式。
2. **文献名称**: *"Phosphorylation-dependent regulation of Smad1 in TGF-β family signaling"*
**作者**: Hata A, Lo RS, Wotton D.
**摘要**: 通过抗磷酸化Smad1(p-Smad1)的小鼠抗体，作者阐明了Smad1在BMP信号转导中的激活机制，并证明其C末端磷酸化对核转位及靶基因调控至关重要。
3. **文献名称**: *"Smad1 knockout mice exhibit defects in angiogenesis"*
**作者**: Goumans MJ, et al.
**摘要**: 研究采用Smad1基因敲除小鼠模型，结合特异性抗体进行免疫荧光染色，证实Smad1缺失导致血管生成异常，揭示其在内皮细胞BMP信号响应中的关键功能。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词 **"Smad1 antibody mouse"** 或 **"Smad1 BMP signaling"**，并筛选方法学中明确使用该抗体的研究。
The Smad1 antibody is a crucial tool in studying the Smad protein family, which plays a central role in transforming growth factor-beta (TGF-β) and bone morphogenetic protein (BMP) signaling pathways. Smad1. a receptor-regulated Smad (R-Smad), is primarily activated by BMP receptors through phosphorylation at C-terminal serine residues. This modification triggers its association with Smad4. facilitating nuclear translocation and regulation of target genes involved in cell differentiation, proliferation, apoptosis, and embryonic development.
Mouse-specific Smad1 antibodies are designed to detect endogenous Smad1 protein in murine tissues or cell lines. These antibodies typically recognize epitopes in the N-terminal or C-terminal regions, with some targeting phosphorylated forms to study activation status. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Validation often includes testing in Smad1-knockout models to confirm specificity.
Research applications span developmental biology, cancer (due to BMP pathway dysregulation), and stem cell studies. Smad1 aberrations are linked to skeletal disorders, vascular diseases, and tumor progression. The antibody's utility extends to both basic research and preclinical models, aiding in understanding Smad1's role in BMP-mediated cellular responses and its crosstalk with other signaling cascades. Proper controls are essential to distinguish Smad1 from closely related Smad5/8 proteins.
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