| WB | 1/4000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Alpha-2Db adrenergic receptor, Alpha-2Db adrenoceptor, Alpha(2Db)AR, Alpha-2Db adrenoreceptor, adra2db |
| Entrez GeneID | 266755 |
| WB Predicted band size | 46.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE adra2db antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 323-356 amino acids from the region of DANRE adra2db. |
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以下是假设性的参考文献示例，基于斑马鱼(Danio rerio)肾上腺素能受体α2D亚型(adra2db)相关研究的合理推测。实际文献需通过学术数据库验证：
1. **"Characterization of α2-adrenergic receptor subtypes in zebrafish embryos"**
*作者：Ruuskanen JO, et al. (2005)*
**摘要**：克隆并鉴定了斑马鱼α2-肾上腺素能受体亚型(包括adra2db)，通过原位杂交和抗体免疫染色揭示了其在神经系统和心脏发育中的表达模式。
2. **"Adra2db modulates cardiac function in zebrafish through catecholamine signaling"**
*作者：Stevens C, et al. (2018)*
**摘要**：利用adra2db特异性抗体进行Western blot和免疫组化，证明该受体在斑马鱼心脏中的功能，调控心率及应激反应。
3. **"CRISPR/Cas9 knockout and antibody-based localization of adra2db in zebrafish larval behavior"**
*作者：Guo S, et al. (2021)*
**摘要**：结合基因编辑与抗体标记技术，揭示adra2db在斑马鱼幼体运动协调和神经递质调控中的关键作用。
4. **"Tissue-specific expression profiling of adrenergic receptors in zebrafish development"**
*作者：Zhang Y, et al. (2019)*
**摘要**：通过qPCR和抗体免疫荧光分析，系统比较了adra2db等受体在胚胎不同组织中的动态表达及功能差异。
**注意**：以上为模拟内容，实际文献需以关键词“zebrafish adra2d antibody”或“Danio rerio alpha2D adrenergic receptor”在PubMed、ZFIN等平台检索。若研究较少，建议扩大至肾上腺素能受体家族或抗体验证方法学文献。
The ADRA2DB antibody is designed to target the adrenoceptor alpha 2D subtype (adra2db) in *Danio rerio* (zebrafish), a model organism widely used in developmental biology and neuropharmacology research. The α2-adrenergic receptors (α2-ARs) are G protein-coupled receptors that mediate physiological responses to catecholamines like norepinephrine and epinephrine, regulating neurotransmitter release, blood pressure, and sympathetic nervous system activity. In zebrafish, the adra2db gene is an ortholog of human ADRA2D, playing roles in cardiovascular function, stress responses, and neurobehavioral regulation.
Zebrafish adra2db is expressed in the central nervous system and peripheral tissues, with studies suggesting its involvement in embryonic development and cardiovascular homeostasis. The ADRA2DB antibody enables researchers to localize and quantify adra2db protein expression, aiding investigations into its functional roles. Its application spans immunohistochemistry, Western blotting, and fluorescence microscopy, particularly in studies exploring adrenergic signaling pathways, drug screening, or genetic models of hypertension and autonomic dysfunction. As zebrafish offer genetic tractability and transparency for in vivo imaging, this antibody serves as a critical tool for dissecting conserved adrenergic mechanisms and modeling human diseases linked to α2-AR dysregulation. Commercial sources often validate it for specificity against zebrafish adra2db, ensuring reliability in cross-species comparative studies.
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