关键信息
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基因名
CTLA4
- 应用
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别名
CTLA4；CD152;Cytotoxic T-lymphocyte Protein 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P16410
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表达区间
37-162aa
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氨基酸序列
AMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCA ATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYP PPYYLGIGNGTQIYVIDPEPCPDSDF
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分子量
14 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is an immune checkpoint receptor that plays a crucial role in regulating T cell-mediated immune responses. It is primarily expressed on the surface of activated T cells and functions as an inhibitory receptor that downregulates immune responses by competing with CD28 for binding to co-stimulatory ligands CD80 (B7-1) and CD86 (B7-2). The discovery of CTLA-4 provided significant insights into immune regulation and has paved the way for the development of immune checkpoint inhibitors in cancer therapy. These inhibitors, which block the activity of CTLA-4, enhance the immune system's ability to target and eliminate tumor cells. Numerous studies have demonstrated that the administration of CTLA-4 antagonists can lead to durable responses in various cancers, including melanoma and non-small cell lung cancer. However, the clinical application of CTLA-4 inhibitors is also associated with immune-related adverse events due to enhanced autoimmunity. Researchers are now focusing on engineering recombinant CTLA-4 proteins to understand their structure-function relationships better, optimize their therapeutic applications, and minimize adverse effects. Additionally, CTLA-4 recombinant proteins are being explored as potential biomarkers for patient stratification in immunotherapy and as tools for dissecting the complex mechanisms of immune regulation. Overall, the study of CTLA-4 and its recombinant proteins is a rapidly evolving field, bridging basic immunology research and advanced cancer treatments, with the potential to significantly improve patient outcomes in oncology.
