Rabbit Polyclonal MouseEpc1 Antibody

The Mouse EPC1 (Eukaryotic Pc1) antibody is designed to detect the endogenous EPC1 protein in mouse tissues and cell lines. EPC1 is a component of the Polycomb Repressive Complex 1 (PRC1), which plays a critical role in epigenetic regulation by mediating chromatin modification and transcriptional silencing. As part of the Polycomb group (PcG) proteins, EPC1 contributes to maintaining repressed chromatin states through histone H2A ubiquitination, a key mechanism for stabilizing gene repression during development and cellular differentiation. The antibody is typically raised in rabbits or other host species using immunogens derived from conserved regions of the mouse EPC1 protein, ensuring specificity for targeting epitopes unique to this isoform. It is widely used in applications such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to study EPC1's role in stem cell maintenance, cancer progression, and developmental biology. Researchers often employ this antibody to explore how dysregulation of PRC1 components, including EPC1. contributes to diseases like leukemia or solid tumors, where Polycomb-mediated gene silencing is frequently disrupted. Validation usually includes testing on knockout controls or siRNA-treated samples to confirm binding specificity.