| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor SOX-2, Sox2, Sox-2 |
| Entrez GeneID | 20674 |
| WB Predicted band size | 34.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Sox2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 34-68 amino acids from the N-terminal region of mouse Sox2. |
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以下是关于Mouse Sox2 (N-term)抗体的参考文献示例(注：文献信息为虚构示例，仅用于演示格式)：
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1. **文献名称**: *Sox2 Expression in Neural Stem Cells: Role of N-terminal Epitope Recognition*
**作者**: Avilion, A.A. et al.
**摘要**: 本研究利用针对Sox2蛋白N端的特异性抗体(Mouse Sox2 N-term)，通过免疫荧光和Western blot分析，揭示了Sox2在维持小鼠神经干细胞多能性中的关键作用。实验验证了抗体在小鼠脑组织中的高特异性和灵敏度。
2. **文献名称**: *Developmental Regulation of Sox2 in Mouse Embryos*
**作者**: Ellis, P. et al.
**摘要**: 通过N端特异性Sox2抗体进行免疫组化分析，本研究系统描述了Sox2在小鼠胚胎早期发育阶段的表达模式，证实其在神经外胚层和生殖细胞谱系中的动态调控。
3. **文献名称**: *Sox2 Interaction with Oct4 in Pluripotency Maintenance*
**作者**: Masui, S. et al.
**摘要**: 使用Mouse Sox2 (N-term)抗体进行免疫共沉淀实验，揭示了Sox2与Oct4在小鼠胚胎干细胞中的直接相互作用，并证明该复合物对多能性相关基因的转录调控至关重要。
4. **文献名称**: *Sox2 Antibody Validation in Glioblastoma Models*
**作者**: Schaffer, A.E. et al.
**摘要**: 本研究评估了多种Sox2抗体的特异性，其中Mouse Sox2 (N-term)抗体在胶质瘤小鼠模型中表现出优异的性能，成功用于流式细胞术和冰冻切片染色，支持其在癌症干细胞研究中的应用。
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**注意**：以上文献为示例，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实文献，并核对抗体货号、实验方法及结论的准确性。
The Mouse Sox2 (N-term) antibody is a monoclonal or polyclonal antibody specifically designed to detect the N-terminal region of the transcription factor Sox2 (SRY-box transcription factor 2). Sox2 is a key regulator of embryonic development and cellular pluripotency, playing a critical role in maintaining the self-renewal capacity of embryonic stem cells (ESCs) and neural progenitor cells. It belongs to the Sox protein family, characterized by a conserved high-mobility group (HMG) DNA-binding domain. Dysregulation of Sox2 is associated with developmental disorders and cancers, particularly in maintaining cancer stem cell populations.
This antibody is raised in mice against a recombinant protein or peptide corresponding to the N-terminal region of Sox2. which helps ensure specificity by minimizing cross-reactivity with other Sox family members (e.g., Sox1. Sox3). It is widely used in applications such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study Sox2 expression in mouse, human, or rat tissues and cell lines. Its validation often includes knockout/knockdown controls to confirm target specificity.
Researchers employ this antibody in stem cell biology, neurodevelopmental studies, and cancer research to explore Sox2's role in cell fate determination, reprogramming, and tumorigenesis. Proper experimental optimization (e.g., dilution ratios, blocking conditions) is recommended to minimize background noise, given Sox2's variable expression levels across cell types.
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