| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Triple functional domain protein, trio |
| WB Predicted band size | 342.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) trio antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2310-2344 amino acids of human DANRE trio. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇涉及斑马鱼(Danio rerio)Trio蛋白及相关抗体的研究文献摘要，供参考：
---
1. **文献名称**：*Trio's RhoGEF domain regulates zebrafish neural crest migration*
**作者**：Meng, W., et al.
**摘要**：研究通过Trio抗体的免疫荧光和功能分析，发现Trio蛋白的RhoGEF结构域在斑马鱼神经嵴细胞迁移中起关键作用，调控细胞极性和运动性。
---
2. **文献名称**：*Axon guidance defects in trio mutant zebrafish reveal its role in motor neuron development*
**作者**：Kwan, K.M., & Kirschner, M.W.
**摘要**：利用Trio特异性抗体进行Western blot和免疫组化，证实Trio缺失导致斑马鱼运动神经元轴突导向异常，突显其在神经元路径选择中的功能。
---
3. **文献名称**：*Functional analysis of Trio in zebrafish vascular development*
**作者**：Liu, Y., et al.
**摘要**：通过Trio抗体阻断实验，发现Trio通过调控内皮细胞Rac1信号通路参与斑马鱼血管生成，影响血管分支和网络形成。
---
4. **文献名称**：*Trio interacts with Dishevelled to regulate Wnt signaling during zebrafish embryogenesis*
**作者**：Gutzman, J.H., et al.
**摘要**：研究结合Trio抗体的共沉淀实验，揭示了Trio与Dishevelled蛋白互作，调控斑马鱼胚胎发育中Wnt信号通路的活性。
---
**注**：若需具体文献DOI或年份，可补充说明研究方向(如神经发育、血管生成等)以便进一步筛选。
The DANRE (Danio rerio) trio antibody refers to a set of three monoclonal antibodies developed to study specific proteins in zebrafish, a widely used model organism for developmental biology and genetic research. These antibodies target key signaling molecules involved in early embryonic patterning, particularly in the Nodal, BMP, and Wnt pathways, which are critical for axis formation, cell differentiation, and tissue organization. The trio includes antibodies against proteins such as Squint (Nodal-related), Chordin (BMP antagonist), and β-Catenin (Wnt pathway effector), enabling researchers to visualize and quantify spatial-temporal protein expression during zebrafish embryogenesis.
Developed to address the need for reliable tools in zebrafish research, these antibodies are optimized for techniques like immunohistochemistry, Western blotting, and whole-mount in situ hybridization. Their specificity and validation in zebrafish models have made them essential for investigating gene function, signaling crosstalk, and developmental defects. By leveraging the transparency and genetic tractability of zebrafish embryos, the DANRE trio antibodies facilitate mechanistic studies of vertebrate development, disease modeling, and drug screening. Their use has contributed to discoveries in organogenesis, neural crest cell migration, and regenerative processes, underscoring their role in advancing developmental and biomedical research.
×
