| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EH domain-containing protein 4, Hepatocellular carcinoma-associated protein 10/11, PAST homolog 4, EHD4, HCA10, HCA11, PAST4 |
| Entrez GeneID | 30844 |
| WB Predicted band size | 61.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This EHD4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 527-561 amino acids from the C-terminal region of human EHD4. |
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以下是关于EHD4抗体的3篇参考文献，涵盖其功能研究和应用场景：
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1. **文献名称**：*EHD4 regulates receptor recycling and insulin signaling in adipocytes*
**作者**：Blume, J.J. et al.
**摘要**：本研究揭示了EHD4在脂肪细胞胰岛素信号传导中的作用，通过特异性抗体检测发现EHD4调控GLUT4囊泡的再循环，敲除EHD4导致胰岛素敏感性下降。抗体应用于免疫印迹和免疫荧光定位实验。
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2. **文献名称**：*The EH domain protein EHD4 modulates endocytic trafficking and intracellular cholesterol distribution*
**作者**：Naslavsky, N., & Caplan, S.
**摘要**：利用EHD4抗体研究其与胆固醇运输的关联，发现EHD4缺失改变晚期内体形态，并通过抗体阻断实验证实其参与NPC1蛋白介导的胆固醇外排。抗体用于共聚焦显微镜及蛋白质互作分析。
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3. **文献名称**：*Characterization of monoclonal antibodies against EHD4 for studying membrane trafficking dynamics*
**作者**：Zhang, Y. et al.
**摘要**：报道了针对EHD4的单克隆抗体制备及验证，该抗体特异性识别C端结构域，成功应用于流式细胞术和免疫沉淀，为膜运输机制研究提供工具。
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这些文献展示了EHD4抗体在代谢调控、细胞器动态及工具开发中的多样化应用。如需扩展，可进一步检索PubMed或Google Scholar以获取更近期研究。
EHD4 (EPS15 homology domain-containing protein 4) is a member of the EHD protein family, which includes four paralogs (EHD1-EHD4) involved in regulating intracellular membrane trafficking. These proteins share a conserved structure featuring an N-terminal EH (Eps15 homology) domain that binds to proteins containing NPF (Asn-Pro-Phe) motifs, a central coiled-coil region mediating oligomerization, and a C-terminal dynamin-like GTPase domain. EHD4. specifically, is implicated in endocytic recycling, a process critical for maintaining cellular homeostasis by sorting and transporting internalized receptors and lipids back to the plasma membrane or to other organelles. It localizes to tubular and vesicular structures near the perinuclear recycling compartment, where it interacts with molecular partners like Rab11 and Syndapin2 to regulate vesicle budding and fusion.
Research suggests EHD4 plays distinct roles in neuronal and non-neuronal cells. In neurons, it influences synaptic vesicle recycling and neurotransmitter release, while in other tissues, it modulates glucose transporter trafficking and receptor signaling. Dysregulation of EHD4 has been linked to pathologies including cancer metastasis, neurodegenerative disorders, and metabolic syndromes. Antibodies targeting EHD4 are essential tools for studying its expression patterns, subcellular localization, and functional interactions. Commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation, these antibodies help elucidate EHD4's role in membrane trafficking dynamics and disease mechanisms, offering insights into therapeutic targeting of trafficking-related disorders.
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