Rabbit Polyclonal MouseItk(N-term) Antibody

The Mouse Itk (N-term) antibody is a monoclonal or polyclonal reagent designed to detect the N-terminal region of interleukin-2-inducible T-cell kinase (Itk), a non-receptor tyrosine kinase belonging to the Tec family. Itk plays a critical role in T-cell receptor (TCR) signaling, regulating processes like T-cell activation, differentiation, and cytokine production. Structurally, Itk contains a pleckstrin homology (PH) domain, a Tec homology (TH) domain, and a kinase domain. The N-terminal region is essential for interactions with adaptor proteins and membrane localization. This antibody is commonly used in immunoblotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) to study Itk expression, post-translational modifications, or signaling dynamics in T-cells. It is particularly valuable in models of autoimmune diseases, allergic responses, and lymphomas, where dysregulated Itk activity is implicated. Validation often includes knockout controls to confirm specificity, with expected molecular weight around 72 kDa. Researchers also employ it to explore Itk's interplay with downstream effectors like PLCγ1 or Vav1. as well as its role in modulating transcriptional regulators (e.g., NFAT). Its application extends to evaluating pharmacological inhibitors targeting Itk in preclinical studies. Proper storage conditions and species reactivity (typically mouse and sometimes cross-reactive with human or rat homologs) are critical considerations for experimental design.