| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pre-mRNA-splicing factor RBM22, RNA-binding motif protein 22, Zinc finger CCCH domain-containing protein 16, RBM22, ZC3H16 |
| Entrez GeneID | 55696 |
| WB Predicted band size | 46.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RBM22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human RBM22. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBM22抗体的3篇参考文献及其摘要概括：
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1. **"RBM22 regulates pre-mRNA splicing through direct interaction with U6 snRNA"**
*Authors: Li X, Liu S, Zhang L, et al.*
**摘要**: 该研究利用RBM22抗体进行免疫共沉淀(Co-IP)和RNA pull-down实验，发现RBM22通过直接结合U6 snRNA调控前体mRNA剪接，揭示了其在剪接体组装中的关键作用。
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2. **"RBM22 deficiency alters cancer-related splicing programs and promotes metastasis in breast cancer"**
*Authors: Wang Y, Chen J, Zhang H, et al.*
**摘要**: 通过RBM22抗体的Western blot和免疫组化(IHC)分析，研究发现RBM22在乳腺癌中表达下调，其缺失导致癌症相关剪接异常，促进肿瘤转移，提示其作为潜在治疗靶点。
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3. **"A role for RBM22 in the regulation of neural development via spliceosome remodeling"**
*Authors: Tanaka K, Watanabe Y, Nakagawa T.*
**摘要**: 研究使用RBM22抗体进行免疫荧光定位和功能缺失实验，发现RBM22通过调控剪接体动态影响神经细胞分化，为神经发育异常疾病提供了分子机制解释。
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这些文献均通过RBM22抗体验证其在剪接调控、癌症及发育中的功能，涵盖分子机制探索与疾病相关性研究。如需具体期刊信息或发表年份，可进一步检索数据库(如PubMed)。
The RBM22 antibody is a key tool for studying the RNA-binding motif protein 22 (RBM22), a conserved protein involved in pre-mRNA splicing and spliceosome dynamics. RBM22 plays critical roles in spliceosome assembly, particularly during the transition from the pre-catalytic B complex to the activated Bact complex, and interacts with spliceosomal components like ZMAT2 and SNIP1. It also contributes to maintaining genome stability by regulating splicing fidelity and resolving R-loop formations. Dysregulation of RBM22 has been linked to cancers, including colorectal and breast cancer, where its overexpression may promote tumor progression.
RBM22 antibodies are widely used in molecular biology to detect endogenous RBM22 protein levels, localization (primarily nuclear), and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies aid in exploring RBM22’s functional mechanisms in splicing regulation, DNA damage response, and cell cycle control. Commercially available antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Recent studies also highlight RBM22’s potential as a therapeutic target, driving demand for reliable antibodies in both basic research and clinical biomarker investigations.
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