Rabbit Polyclonal (DANRE)rfx4 Antibody

The RFX4 antibody is a tool used to study Regulatory Factor X4 (RFX4), a transcription factor belonging to the RFX family, which binds DNA via a winged-helix domain. RFX4 plays critical roles in vertebrate development, particularly in the central nervous system (CNS) and ciliated tissues. In zebrafish (*Danio rerio*, abbreviated as *Danio rerio* or DANRE in some databases), RFX4 is implicated in neurogenesis, ciliogenesis, and left-right patterning. Studies in model organisms, including zebrafish, have shown that RFX4 regulates genes involved in cilia assembly and function, and its dysfunction is linked to congenital hydrocephalus and other developmental defects. The RFX4 antibody is typically generated against conserved epitopes, enabling detection across species. It is utilized in techniques like immunohistochemistry, Western blotting, and immunofluorescence to localize RFX4 expression in tissues, monitor protein levels during development, or validate genetic models (e.g., CRISPR/Cas9 knockouts). Research using this antibody has helped clarify RFX4's role in maintaining neural progenitor cells and coordinating cilia-dependent signaling pathways. Its specificity is often validated via knockout controls or peptide blocking assays. Commercial and custom-made RFX4 antibodies are available, with applications spanning developmental biology, neurobiology, and disease modeling.