Rabbit Polyclonal (DANRE)afmid(N-term) Antibody

The DANRE afmid (N-term) antibody is a specialized tool designed to detect the N-terminal region of the arylformamidase (afmid) protein in *Danio rerio* (zebrafish). Afmid, encoded by the *afmid* gene, is an enzyme involved in the kynurenine pathway, which plays a critical role in tryptophan metabolism and the regulation of neuroactive metabolites. In zebrafish models, this pathway is studied for its implications in neurological development, immune responses, and diseases such as neurodegeneration or cancer. The antibody's specificity for the N-terminal region ensures targeted recognition of full-length or truncated afmid variants, aiding in studies of protein expression, localization, and function. It is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to visualize afmid in tissue samples or embryos. Researchers employ this antibody to explore afmid's role in developmental biology, toxicology, or metabolic disorders, leveraging zebrafish as a model organism due to its genetic tractability and translucency during early stages. Validation typically includes reactivity checks in zebrafish lysates and knockout controls to confirm specificity. This tool thus supports mechanistic insights into metabolic pathways and their dysregulation in disease contexts.