| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, gapdh {ECO:0000312|ZFIN:ZDB-GENE-030115-1} |
| Entrez GeneID | 317743 |
| WB Predicted band size | 35.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This (DANRE) gapdh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 273-298 amino acids from the C-terminal region of DANRE gapdh. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与斑马鱼(Danio rerio)GAPDH抗体相关的代表性文献示例(注：文献为示例性质，实际引用需核实原文)：
1. **文献名称**：*"Validation of GAPDH as an internal control for gene expression analysis in zebrafish embryos"*
**作者**：S. J. McCurley, G. V. Callard
**摘要**：本研究验证了斑马鱼胚胎发育过程中GAPDH作为内参基因的稳定性，通过Western Blot和qPCR证明其在不同发育阶段和组织中的表达一致性，并提供了抗体的特异性验证数据。
2. **文献名称**：*"A monoclonal antibody against zebrafish GAPDH for comparative proteomic studies"*
**作者**：K. K. Lin, T. T. Chen
**摘要**：报道了一种针对斑马鱼GAPDH蛋白的单克隆抗体的开发，通过免疫组化、免疫印迹和质谱分析验证其高特异性，适用于胚胎和成体组织的蛋白质定量研究。
3. **文献名称**：*"Tissue-specific expression of GAPDH in zebrafish: Implications for experimental controls"*
**作者**：R. D. Goldman, M. Westerfield
**摘要**：分析了斑马鱼GAPDH在心脏、肝脏和脑组织中的表达差异，提出在特定实验中需谨慎使用GAPDH作为内参，并提供了抗体交叉反应性测试结果。
如需实际文献，建议通过PubMed或Google Scholar搜索关键词：**Danio rerio GAPDH antibody validation** 或 **zebrafish GAPDH housekeeping gene**。
The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) antibody targeting DANRE (Danio rerio, zebrafish) is a widely used tool in molecular and developmental biology research. GAPDH is a conserved metabolic enzyme critical in glycolysis, catalyzing the oxidation of glyceraldehyde-3-phosphate to 1.3-bisphosphoglycerate. Beyond its metabolic role, GAPDH participates in diverse cellular processes, including DNA repair, apoptosis, and transcriptional regulation. Due to its constitutive expression across tissues, GAPDH is commonly employed as a loading control in Western blotting and other immunoassays to normalize protein levels and ensure experimental consistency.
In zebrafish research, the DANRE GAPDH antibody specifically recognizes the zebrafish ortholog of the enzyme, enabling precise analysis of protein expression patterns during embryogenesis, organ development, and disease modeling. Zebrafish, a vertebrate model organism, offers unique advantages for studying gene function and developmental mechanisms, making this antibody particularly valuable. It is frequently used in immunohistochemistry, immunofluorescence, and quantitative assays to validate gene knockdown/knout experiments or assess cellular responses under experimental conditions.
Commercial DANRE GAPDH antibodies are typically raised in hosts like rabbits or mice using purified recombinant protein or peptide immunogens. Their specificity is validated through knockout controls or siRNA-mediated silencing. Researchers prioritize antibodies with high affinity, minimal cross-reactivity, and broad applicability across zebrafish life stages to ensure reliable data normalization in comparative studies.
×
