| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK |
| Entrez GeneID | 8877 |
| WB Predicted band size | 42.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是关于SPHK1(N-term P74)抗体的3篇参考文献示例(注：文献为虚构示例，实际需根据具体数据库检索)：
---
1. **文献名称**: "Characterization of a Novel Anti-SPHK1 Antibody Targeting the N-terminal Domain"
**作者**: Smith A, et al.
**摘要**: 本研究开发并验证了一种针对SPHK1蛋白N端P74表位的高特异性单克隆抗体。通过免疫印迹和免疫组化实验证实该抗体在多种细胞系中可特异性识别SPHK1.并用于探究其在细胞增殖和凋亡中的作用。
---
2. **文献名称**: "SPHK1 N-terminal Phosphorylation Regulates Enzymatic Activity in Cancer Cells"
**作者**: Lee B, et al.
**摘要**: 利用抗SPHK1(N-term P74)抗体，研究者发现SPHK1的N端磷酸化修饰(P74位点)对其激酶活性至关重要。该抗体被用于共免疫沉淀实验，揭示了SPHK1与PI3K/Akt通路的相互作用机制。
---
3. **文献名称**: "Development of Polyclonal Antibodies Against SPHK1 for Diagnostic Applications"
**作者**: Chen X, et al.
**摘要**: 本研究报道了一种针对SPHK1 N端区域的兔源多克隆抗体的制备与验证。该抗体(识别P74表位)在乳腺癌组织样本中显示高灵敏度和特异性，提示其在临床病理检测中的潜在应用价值。
---
如需真实文献，建议在PubMed或Google Scholar中检索关键词：
`SPHK1 antibody N-terminal`, `anti-SPHK1 P74 epitope`, `SPHK1 phosphorylation antibody`。
The SPHK1 (N-term P74) antibody is a specialized tool designed to target the N-terminal region of sphingosine kinase 1 (SPHK1), specifically recognizing a phosphorylation site at position 74 (P74). SPHK1 is a key enzyme in the sphingolipid metabolism pathway, catalyzing the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), a bioactive lipid mediator involved in critical cellular processes such as proliferation, survival, migration, and immune regulation. Dysregulation of SPHK1/S1P signaling has been linked to pathologies including cancer, inflammation, and metabolic disorders. The phosphorylation of SPHK1 at P74 is associated with its enzymatic activation and subcellular localization, making this site functionally significant.
The SPHK1 (N-term P74) antibody is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study SPHK1 expression, activation, and regulatory mechanisms. Its specificity for the phosphorylated P74 residue allows researchers to differentiate between active and inactive forms of SPHK1. providing insights into its role in disease contexts. This antibody has been particularly valuable in cancer research, where SPHK1 overexpression correlates with tumor progression and therapy resistance, and in exploring inflammatory pathways. By enabling precise detection of SPHK1 activation states, it supports the development of targeted therapies aimed at modulating SPHK1 activity in pathological conditions.
×
