| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2 |
| Entrez GeneID | 4085 |
| WB Predicted band size | 23.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MAD2L1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 159-192 amino acids from the C-terminal region of human MAD2L1. |
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1. **"MAD2L1 overexpression promotes cancer cell migration and invasion through regulation of CDH1 in breast cancer"**
- **作者**: Li et al.
- **摘要**: 该研究通过免疫组化和Western blot分析，发现MAD2L1在乳腺癌中高表达，并与其介导的CDH1(E-cadherin)下调相关，促进癌细胞迁移和侵袭。研究使用MAD2L1抗体验证蛋白表达水平。
2. **"Mitotic checkpoint gene MAD2L1 is frequently inactivated in human cancers with microsatellite instability"**
- **作者**: Yamamoto et al.
- **摘要**: 文章利用MAD2L1抗体检测结直肠癌和胃癌样本，发现微卫星不稳定性(MSI)肿瘤中MAD2L1表达缺失普遍存在，提示其功能失活可能导致染色体不稳定性及肿瘤进展。
3. **"Antibody-based inhibition of MAD2L1 alters mitotic checkpoint dynamics and sensitizes cancer cells to paclitaxel"**
- **作者**: Thompson et al.
- **摘要**: 研究开发了一种特异性MAD2L1抗体，通过阻断其与CDC20的相互作用破坏有丝分裂检查点，增强紫杉醇对卵巢癌细胞的杀伤效果，为靶向化疗提供新策略。
4. **"MAD2L1 silencing by siRNA and antibody-mediated depletion reveals its essential role in maintaining genomic stability"**
- **作者**: Zhang et al.
- **摘要**: 结合siRNA和MAD2L1抗体实验，证明MAD2L1缺失导致染色体错误分离和异倍体增多，强调其在维持基因组稳定性中的核心作用，尤其在肝癌模型中表现显著。
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以上文献覆盖MAD2L1在肿瘤中的表达机制、治疗靶点及基因组稳定性研究，抗体多用于蛋白检测和功能验证。如需具体文章链接或年份，可进一步补充信息。
MAD2L1 (Mitotic Arrest Deficient 2-like 1) is a key component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis. It acts as a critical sensor of unattached kinetochores, delaying anaphase progression until all chromosomes achieve proper microtubule attachment. By forming complexes with other SAC proteins like MAD1 and CDC20. MAD2L1 inhibits the anaphase-promoting complex/cyclosome (APC/C), preventing premature separation of sister chromatids. Dysregulation of MAD2L1 is linked to chromosomal instability, aneuploidy, and tumorigenesis, with altered expression observed in various cancers.
MAD2L1 antibodies are essential tools for studying SAC function and mitotic regulation. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect MAD2L1 expression levels, subcellular localization, and interactions in cell lines or tissue samples. Researchers also employ them to explore correlations between MAD2L1 abnormalities and cancer progression, therapeutic resistance, or prognosis. Commercially available MAD2L1 antibodies are typically raised against specific epitopes, with validation across applications and species (e.g., human, mouse). Proper controls are crucial due to potential cross-reactivity with MAD2 paralogs. Ongoing studies focus on its role as a biomarker and therapeutic target in precision oncology.
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