| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XIAP-associated factor 1, BIRC4-binding protein, XAF1, BIRC4BP, XIAPAF1 |
| Entrez GeneID | 54739 |
| WB Predicted band size | 34.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This XAF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 235-269 amino acids from the C-terminal region of human XAF1. |
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以下是关于XAF1抗体的3篇代表性文献的简要总结(文献信息为模拟示例，实际引用需核实)：
1. **文献名称**：*XAF1 modulates apoptotic cell death and proliferation in colorectal cancer*
**作者**：Li L, et al.
**摘要**：本研究通过免疫组化(使用XAF1特异性抗体)分析结直肠癌组织中XAF1的表达，发现其低表达与肿瘤进展和化疗耐药相关，提示XAF1通过调控凋亡通路抑制癌细胞生长。
2. **文献名称**：*Development of a monoclonal antibody against XAF1 for functional studies in apoptosis*
**作者**：Wang Y, et al.
**摘要**：报道了一种新型抗人XAF1单克隆抗体的开发，验证了其在Western blot和免疫荧光中的应用，并证明该抗体可有效检测XAF1与XIAP的相互作用，为凋亡机制研究提供工具。
3. **文献名称**：*XAF1 as a prognostic biomarker in gastric cancer: immunohistochemical analysis*
**作者**：Kim S, et al.
**摘要**：利用XAF1抗体对胃癌组织进行染色，发现XAF1高表达患者生存期显著延长，表明其可作为预后标志物，并可能通过促进癌细胞凋亡抑制肿瘤转移。
**提示**：建议通过PubMed或Google Scholar检索最新文献，关键词“XAF1 antibody”或“XAF1 immunohistochemistry”，以获取具体实验方法和应用场景的详细信息。
The X-linked inhibitor of apoptosis-associated factor 1 (XAF1) antibody is a tool used to study the XAF1 protein, a tumor suppressor implicated in apoptosis regulation. XAF1 was initially identified as a binding partner of XIAP (X-linked inhibitor of apoptosis protein), counteracting its anti-apoptotic activity by promoting XIAP degradation or sequestering it from caspase inhibition. Structurally, XAF1 is a zinc-finger protein encoded by a seven-exon gene, expressed in various tissues but frequently downregulated in cancers, including colorectal, gastric, and breast malignancies, often due to promoter hypermethylation.
XAF1 antibodies enable researchers to detect and quantify XAF1 expression in cells or tissues, aiding investigations into its role in apoptosis, tumor suppression, and chemotherapy resistance. Studies show XAF1 induces apoptosis via both XIAP-dependent and independent pathways, including p53 activation and MAPK signaling. Its expression correlates with improved patient survival in certain cancers, highlighting its prognostic value. Additionally, XAF1 antibodies help explore interactions with viral proteins, as XAF1 also exhibits antiviral properties by modulating interferon signaling.
Research applications include immunohistochemistry, Western blotting, and flow cytometry to assess XAF1 dynamics in normal vs. cancerous tissues or during therapeutic interventions. These studies contribute to understanding XAF1's dual role in apoptosis and innate immunity, supporting its potential as a biomarker or therapeutic target.
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