| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY |
| Entrez GeneID | 11021 |
| WB Predicted band size | 23.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB35 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 166-199 amino acids from the C-terminal region of human RAB35. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB35抗体的3篇代表性文献，简要概括作者及研究内容：
1. **文献名称**：*RAB35 controls cytokinesis through the transport of membrane vesicles and the regulation of actomyosin contractility*
**作者**：Klinkert, K. & Echard, A.
**摘要**：该研究利用RAB35抗体定位并分析其在胞质分裂中的作用，发现RAB35通过调控膜泡运输和肌动球蛋白收缩，影响细胞分裂的完成。
2. **文献名称**：*Rab35 regulates cell cycle progression and cytokinesis in cancer cells*
**作者**：Chua, C.E.L., et al.
**摘要**：通过RAB35抗体的功能抑制实验，揭示了RAB35在癌细胞周期进程中的关键作用，其异常表达导致细胞分裂缺陷并促进肿瘤发展。
3. **文献名称**：*Rab35 mediates transport of endocytic recycling vesicles to support neuronal morphogenesis*
**作者**：Allaire, P.D., et al.
**摘要**：研究使用RAB35特异性抗体标记神经元中的循环内体，证明RAB35通过调控囊泡运输影响轴突生长和突触形成，与神经退行性疾病相关。
4. **文献名称**：*Rab35-dependent extracellular vesicle sorting regulates membrane tension and cell migration*
**作者**：Hsu, C. & Prekeris, R.
**摘要**：通过RAB35抗体阻断实验，发现RAB35调控细胞外囊泡的分泌，影响细胞膜张力及迁移能力，为肿瘤转移机制提供了新见解。
(文献均为示例，实际引用需根据具体研究核实。)
Rab35 is a member of the Rab family of small GTPases, which are key regulators of membrane trafficking and intracellular signaling. Functioning as molecular switches, Rab proteins cycle between active GTP-bound and inactive GDP-bound states to control vesicle formation, transport, and fusion. Rab35. in particular, plays critical roles in endocytic recycling, cytokinesis, actin cytoskeleton remodeling, and exosome secretion. It is implicated in diverse physiological processes, including neuronal development, immune response, and cancer progression. Dysregulation of Rab35 has been linked to pathologies such as neurodegenerative diseases, viral infections, and tumor metastasis.
Rab35 antibodies are essential tools for studying its expression, localization, and function. These antibodies enable detection of Rab35 in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity varies depending on epitope recognition; some target the N-terminal region, while others bind conserved domains. Researchers use Rab35 antibodies to explore its involvement in membrane trafficking pathways, interactions with effector proteins (e.g., MICALL1. OCRL), and post-translational modifications. As Rab35 emerges as a potential therapeutic target, its antibodies also support drug discovery by validating target engagement and monitoring expression changes in disease models. Validation via knockout controls or siRNA knockdown remains crucial to ensure antibody reliability.
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