首页 / 产品 / 抗体 / 一抗

Rabbit Polyclonal PHF1(N-term) Antibody

  • 中文名： PHF1 (N-term)抗体
  • 别    名： PHD finger protein 1, Protein PHF1, hPHF1, Polycomb-like protein 1, hPCl1, PHF1, PCL1
货号: IPDX32665
Price： ￥1180
数量：
大包装询价

验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPHD finger protein 1, Protein PHF1, hPHF1, Polycomb-like protein 1, hPCl1, PHF1, PCL1
Entrez GeneID5252
WB Predicted band size62.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenThis PHF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 164-197 amino acids from the N-terminal region of human PHF1.

+ +

参考文献

以下是关于PHF1(N-term)抗体的3篇参考文献示例(内容为模拟，仅供参考)：

---

1. **文献名称**: "PHF1 regulates H3K27 methylation and neural differentiation through PRC2 complex assembly"

**作者**: Li, Y., et al. (2017)

**摘要**: 本研究利用PHF1(N-term)抗体进行免疫沉淀和Western blot分析，揭示了PHF1通过稳定PRC2复合物调控H3K27三甲基化，并影响神经干细胞分化。

2. **文献名称**: "A novel role for PHF1 in DNA damage response via interaction with BRCA1"

**作者**: Zhang, H., et al. (2019)

**摘要**: 通过PHF1(N-term)抗体的ChIP-seq和免疫荧光实验，作者发现PHF1的N端结构域与BRCA1结合，参与DNA损伤修复通路。

3. **文献名称**: "PHF1 expression is upregulated in endometrial cancer and promotes tumor progression"

**作者**: Chen, J., et al. (2020)

**摘要**: 使用PHF1(N-term)抗体进行组织免疫组化分析，证明PHF1在子宫内膜癌中高表达，并通过激活Wnt信号通路促进肿瘤侵袭。

---

如需真实文献，建议通过PubMed或Google Scholar搜索关键词“PHF1 antibody N-terminal”，并筛选应用类文章;也可参考抗体厂商(如CST、Abcam)的产品引用列表。

背景信息

The PHF1 (N-term) antibody is a specialized tool used to detect the N-terminal region of the Plant Homeodomain Finger Protein 1 (PHF1), a chromatin-associated protein involved in epigenetic regulation. PHF1. also known as PCL1. is a core component of the Polycomb Repressive Complex 2 (PRC2), which mediates histone H3 lysine 27 trimethylation (H3K27me3), a key epigenetic mark for gene silencing. The N-terminal domain of PHF1 contains conserved PHD fingers critical for chromatin interaction and PRC2 recruitment to specific genomic loci. This antibody is commonly utilized in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to study PHF1 expression, localization, and its role in gene regulation. Research highlights its involvement in developmental processes, DNA repair, and tumor suppression. Dysregulation of PHF1 is linked to cancers and genetic disorders, such as endometrial stromal sarcomas (where PHF1 fusions occur) and midline carcinomas. Validated for specificity, the PHF1 (N-term) antibody helps dissect molecular mechanisms underlying epigenetic silencing, cellular differentiation, and disease pathogenesis, making it valuable in both basic and translational research contexts.

客户数据及评论

折叠内容

大包装询价

×