Rabbit Polyclonal GNGT1 Antibody

中文名： GNGT1抗体
别名： Guanine nucleotide-binding protein G(T) subunit gamma-T1, Transducin gamma chain, GNGT1

货号: IPDX32662

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Guanine nucleotide-binding protein G(T) subunit gamma-T1, Transducin gamma chain, GNGT1
Entrez GeneID	2792
WB Predicted band size	8.5kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This GNGT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 56-91 amino acids from the C-terminal region of human GNGT1.

+ +

Western blot analysis of lysate from mouse eyeball tissue lysate, using GNGT1 Antibody (C-term)(Cat. #P32662). P32662 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

参考文献

以下是关于GNGT1抗体的3篇参考文献示例(注：内容为模拟虚构，仅供参考)：

1. **标题**: "GNGT1 regulates light-dependent signaling in retinal photoreceptors"

**作者**: Lee, J. et al.

**摘要**: 本研究利用GNGT1特异性抗体，通过免疫荧光和Western blot技术，揭示了GNGT1在视网膜视杆细胞中的高表达，并证实其参与光信号转导通路中cGMP的调控。

2. **标题**: "Characterization of a polyclonal antibody against human GNGT1 for functional studies"

**作者**: Zhang, Y. & Smith, R.

**摘要**: 文章报道了一种新型多克隆抗体的开发，该抗体针对人源GNGT1蛋白C末端肽段，经验证可用于免疫沉淀及组织定位，为研究G蛋白偶联受体信号机制提供工具。

3. **标题**: "GNGT1 knockout mice exhibit visual impairment and altered retinal morphology"

**作者**: Chen, H. et al.

**摘要**: 通过GNGT1抗体检测基因敲除小鼠视网膜组织，发现GNGT1缺失导致光敏蛋白分布异常，提示其在视觉发育中的关键作用。

4. **标题**: "Antibody validation for G-protein gamma subunits in model organisms"

**作者**: Wilson, K. et al.

**摘要**: 本研究系统评估了GNGT1抗体在斑马鱼和小鼠中的交叉反应性，确认其在进化保守表位上的特异性，支持跨物种比较研究。

建议通过PubMed或Google Scholar检索真实文献，或查阅抗体供应商(如Abcam、Sigma-Aldrich)提供的产品引用文献。

背景信息

GNGT1 (G Protein Subunit Gamma Transducin 1) is a member of the G protein gamma subunit family, which plays a critical role in G protein-coupled receptor (GPCR) signaling pathways. G proteins, composed of alpha, beta, and gamma subunits, mediate cellular responses to extracellular signals by transmitting signals from activated GPCRs to intracellular effectors. Specifically, GNGT1 pairs with the beta subunit to form the βγ complex, regulating downstream effectors such as ion channels, enzymes, and second messengers.

The GNGT1 protein is highly expressed in retinal photoreceptor cells, where it associates with transducin (a G protein alpha subunit) to facilitate phototransduction—the process converting light into electrical signals. Dysregulation of GNGT1 has been implicated in visual disorders, including congenital stationary night blindness and retinal degeneration. Beyond the retina, GNGT1 is found in other tissues, suggesting broader roles in cellular signaling.

Antibodies targeting GNGT1 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate GPCR signaling mechanisms, tissue-specific roles, and disease associations. Research utilizing GNGT1 antibodies contributes to understanding pathological conditions linked to GPCR dysfunction, such as cancer, neurological disorders, and metabolic diseases. These antibodies also aid in validating gene-editing outcomes and exploring therapeutic targets in precision medicine.