Mouse Monoclonal RPS6(N-term) Antibody

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     Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term)(Cat.  #P32631).  P32631 was diluted at 1:2000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.    

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     Fluorescent image of Hela cells stained with RPS6 Antibody (N-term)(Cat#P32631).  P32631 was diluted at 1:25 dilution.  An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green).  Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).    

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     Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green, Cat#P32631) compared to an isotype control of mouse IgG1(blue).  P32631 was diluted at 1:25 dilution.  An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.    

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     Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term)(Cat#P32631). P32631 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term)(Cat#P32631). P32631 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term)(Cat#P32631). P32631 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term)(Cat#P32631). P32631 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.    

The RPS6 (N-term) antibody is designed to target the N-terminal region of ribosomal protein S6 (RPS6), a component of the 40S ribosomal subunit involved in mRNA translation and cellular homeostasis. RPS6 plays a critical role in regulating protein synthesis, cell growth, proliferation, and stress responses. It is a downstream effector of the mTOR (mechanistic target of rapamycin) signaling pathway, where phosphorylation of specific serine residues (e.g., Ser235/236) serves as a biomarker for mTORC1 activity. The N-terminal region of RPS6 contains conserved domains essential for ribosome assembly and interaction with other ribosomal proteins or regulatory factors.

This antibody is widely used in research to study RPS6 expression, post-translational modifications (e.g., phosphorylation), and subcellular localization in various biological contexts. It is commonly applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate mTOR pathway activation, cellular responses to nutrients, growth factors, or stress conditions. Dysregulation of RPS6 has been implicated in cancers, metabolic disorders, and neurodegenerative diseases, making this antibody a valuable tool for exploring disease mechanisms. Validation typically includes specificity checks across species (human, mouse, rat) and confirmation of reactivity in knockout controls. Its utility extends to both basic research and preclinical studies targeting mTOR-related pathways.