| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1 |
| Entrez GeneID | 1027 |
| WB Predicted band size | 22.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CDKN1B antibody is generated from a mouse immunized with CDKN1B recombinant protein. |
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以下是关于CDKN1B抗体的3篇参考文献示例：
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1. **文献名称**: "p27(Kip1) (CDKN1B) expression in human breast cancer: prognostic implications and clinicopathological associations"
**作者**: Tan P. et al.
**摘要**: 该研究通过免疫组化分析乳腺癌组织中CDKN1B/p27蛋白的表达水平，发现其低表达与肿瘤进展和患者生存率降低显著相关，提示CDKN1B可作为乳腺癌预后标志物。
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2. **文献名称**: "Antibody-based detection of CDKN1B/p27Kip1 in colorectal cancer: validation and clinical utility"
**作者**: Müller H. et al.
**摘要**: 研究者验证了一种特异性CDKN1B抗体的灵敏度和特异性，发现其在结直肠癌组织中的检测可区分不同分子亚型，并证实p27表达缺失与转移风险升高相关。
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3. **文献名称**: "Genetic alterations and protein expression of CDKN1B in gastric carcinogenesis"
**作者**: Li Y. et al.
**摘要**: 通过Western blot和免疫组化结合基因测序，发现胃癌中CDKN1B基因突变和启动子甲基化导致蛋白表达下调，提示其在胃癌发生中的抑癌作用。
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4. **文献名称**: "CDKN1B/p27 polymorphism and antibody-based quantification in prostate cancer progression"
**作者**: Chen J. et al.
**摘要**: 研究利用特异性抗体分析前列腺癌组织中p27的亚细胞定位，发现胞质内异常积聚与肿瘤侵袭性相关，为治疗靶点选择提供依据。
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以上文献聚焦于CDKN1B抗体在肿瘤研究中的应用，涵盖表达分析、预后评估及分子机制探索。
The CDKN1B antibody is a crucial tool in studying the cell cycle regulator p27Kip1. a protein encoded by the CDKN1B gene. p27Kip1 belongs to the CIP/KIP family of cyclin-dependent kinase (CDK) inhibitors, which regulate cell cycle progression by binding to and inhibiting cyclin-CDK complexes, particularly during the G1-to-S phase transition. This protein plays a pivotal role in controlling cellular proliferation, differentiation, and apoptosis, making it a focus in cancer research, as its dysregulation is linked to tumorigenesis and poor prognosis in various cancers.
CDKN1B antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect p27Kip1 expression levels and subcellular localization. These applications help researchers assess its role in cell cycle arrest, where nuclear p27Kip1 acts as a tumor suppressor, versus cytoplasmic localization, which is associated with cancer aggressiveness. Additionally, the antibody aids in studying post-translational modifications (e.g., phosphorylation, ubiquitination) that regulate p27Kip1 stability and activity.
Commercial CDKN1B antibodies are typically validated for specificity using knockout cell lines or siRNA knockdowns. Their utility extends to both basic research and clinical studies, particularly in evaluating biomarkers for cancer diagnosis and therapeutic targeting. Challenges include cross-reactivity with homologous proteins (e.g., p21. p57) and variability in detection due to tissue-specific epitopes or fixation methods. Overall, CDKN1B antibodies remain indispensable for unraveling the complex roles of p27Kip1 in health and disease.
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