| WB | 1/1000 | Human, Drosophila |
| IF | 咨询技术 | Human, Drosophila |
| IHC | 咨询技术 | Human, Drosophila |
| ICC | 技术咨询 | Human, Drosophila |
| FCM | 咨询技术 | Human, Drosophila |
| Elisa | 咨询技术 | Human, Drosophila |
| Entrez GeneID | 43490 |
| WB Predicted band size | 37.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Drosophila |
| Immunogen | This BUB3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from Drosophila melanogaster (Fruit fly) BUB3. |
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以下是关于果蝇BUB3抗体的3篇参考文献及其摘要概括：
1. **"Drosophila Bub3 is required for kinetochore localization of BubR1 and spindle checkpoint function"**
*作者：Lopes CS et al.*
摘要：研究通过免疫荧光实验，利用果蝇BUB3抗体证实了BUB3在细胞分裂过程中对BubR1定位的调控作用，并揭示其纺锤体检查点功能缺陷导致的染色体分离异常。
2. **"The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in Drosophila"**
*作者：Meraldi P et al.*
摘要：文献通过Western blot和免疫沉淀技术，使用BUB3抗体证明果蝇中BUB3与微管结合蛋白的相互作用，并探讨其在染色体稳定性维持中的分子机制。
3. **"Functional analysis of the Drosophila mitotic checkpoint proteins"**
*作者：Basu J et al.*
摘要：研究利用果蝇BUB3抗体进行组织特异性敲低实验，发现BUB3缺失导致纺锤体检查点失效，并引发胚胎发育异常，强调其在细胞周期调控中的必要性。
(注：以上文献名为虚拟示例，实际文献需通过PubMed或Google Scholar按关键词检索。)
The BUB3 protein in *Drosophila melanogaster* plays a critical role in the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis. BUB3 is part of a conserved protein complex, including Bub1 and BubR1. which monitors kinetochore-microtubule attachments and delays anaphase onset until all chromosomes are properly aligned. This checkpoint prevents aneuploidy, a hallmark of genomic instability linked to developmental disorders and cancer. In *Drosophila*, BUB3 is essential for maintaining genome integrity during cell division, particularly in rapidly dividing tissues like larval brain cells and imaginal discs.
Antibodies targeting *Drosophila* BUB3 are vital tools for studying SAC function, cell cycle regulation, and chromosome dynamics in genetic and developmental models. These antibodies enable researchers to localize BUB3 via immunofluorescence, assess protein expression levels through Western blotting, and investigate interactions with other checkpoint components. Given the evolutionary conservation of SAC proteins, studies in *Drosophila* using BUB3 antibodies often provide insights into analogous mechanisms in vertebrates. Additionally, such antibodies help explore BUB3's role in DNA damage responses, meiotic divisions, and its potential dysregulation in disease contexts. Validated specificity for *Drosophila* BUB3 ensures minimal cross-reactivity with related proteins in other species, making them indispensable for dissecting SAC pathways in this model organism.
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