Mouse Monoclonal ALDH2 Antibody

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     Western blot analysis of lysates from NCI-H292, A549, HepG2 cell line (from left to right), using ALDH2 Antibody(Cat.  #P32621).  P32621 was diluted at 1:1000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.    

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     Confocal immunofluorescent analysis of ALDH2 Antibody(Cat.  #P32621)with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).    

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     ALDH2 Monoclonal Antibody (Cat. #P32621) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.    

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     ALDH2 Monoclonal Antibody (Cat. #P32621) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.  This data demonstrates the use of the ALDH2 Monoclonal Antibody for immunohistochemistry.  Clinical relevance has not been evaluated.    

Aldehyde dehydrogenase 2 (ALDH2) is a mitochondrial enzyme critical for detoxifying reactive aldehydes, including acetaldehyde (a carcinogenic byproduct of ethanol metabolism) and lipid peroxidation-derived aldehydes. It plays a key role in cellular defense against oxidative stress and metabolic disorders. The ALDH2 gene exhibits a common polymorphism (rs671. Glu504Lys), prevalent in East Asian populations, which significantly reduces enzyme activity. This variant is linked to "alcohol flush reaction," increased cancer risk (e.g., esophageal and gastric cancers), and susceptibility to cardiovascular diseases (e.g., coronary artery disease) and neurodegenerative conditions.

ALDH2 antibodies are essential tools for studying protein expression, localization, and function in research. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess ALDH2 levels in tissues or cells, particularly in studies exploring metabolic syndromes, alcohol-related pathologies, and oxidative stress mechanisms. Commercially available antibodies are typically raised against specific epitopes of human ALDH2. with validation across species (e.g., human, mouse, rat). Researchers prioritize antibodies validated for specificity through knockout controls or siRNA silencing to avoid cross-reactivity with other ALDH isoforms. These reagents are vital for elucidating ALDH2's role in disease progression and potential therapeutic targeting.