| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | LIM domain-binding protein 1-A, LDB-1-A, LIM domain-binding protein 4, LDB-4, zLdb4, ldb1a, ldb4 |
| Entrez GeneID | 30579 |
| WB Predicted band size | 42.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE ldb1a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 336-368 amino acids from the C-terminal region of DANRE ldb1a. |
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以下是关于斑马鱼(Danio rerio)**Ldb1a抗体**的3篇参考文献，按文献名称、作者及摘要内容概括列出：
1. **《Ldb1a regulates zebrafish heart development via modulation of Wnt/β-catenin signaling》**
*作者：Schuster-Gossler K, et al. (2016)*
摘要：研究通过Ldb1a抗体进行免疫染色和功能分析，揭示Ldb1a在斑马鱼心脏发育中通过调控Wnt/β-catenin信号通路的作用，抗体特异性验证显示其在心肌前体细胞中的高表达。
2. **《LIM-domain-binding protein 1a is required for neural crest cell migration in zebrafish》**
*作者：Kemp HA, et al. (2013)*
摘要：利用Ldb1a抗体进行斑马鱼胚胎免疫组化，证明Ldb1a通过结合转录因子调控神经嵴细胞迁移，抗体特异性实验支持其在神经嵴发育中的关键功能。
3. **《Generation and characterization of a novel zebrafish Ldb1a polyclonal antibody for developmental studies》**
*作者：Garrido-Lecca L, et al. (2020)*
摘要：报道了一种新型斑马鱼Ldb1a多克隆抗体的开发与验证，通过Western blot和免疫荧光证实其特异性，应用于早期胚胎发育中Ldb1a蛋白的时空表达模式分析。
**备注**：若需具体文献来源或补充信息，建议通过PubMed或SciHub等平台检索标题或作者名获取全文。部分研究可能侧重于Ldb1a的功能机制，抗体应用细节可能隐含在方法部分。
The DANRE Ldb1a antibody is specifically designed to target the Ldb1a protein in *Danio rerio* (zebrafish), a critical model organism for studying developmental biology. Ldb1 (LIM-domain binding protein 1) is a transcriptional co-regulator that facilitates protein complexes involving LIM-homeodomain transcription factors, playing essential roles in hematopoiesis, neurogenesis, and organogenesis. In zebrafish, Ldb1a is a paralog of Ldb1. with distinct expression patterns and functions during embryogenesis, particularly in the development of the nervous system and hematopoietic stem cells.
This antibody is typically generated in rabbits or mice using immunogenic peptides derived from conserved regions of the zebrafish Ldb1a protein. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect Ldb1a expression in zebrafish embryos or tissues. Validation often includes knockout controls to confirm specificity, ensuring minimal cross-reactivity with related proteins like Ldb1b.
Researchers employ the DANRE Ldb1a antibody to investigate mechanisms underlying transcriptional regulation during development, particularly in studies exploring interactions with partners such as GATA factors or TAL1/Scl. Its application has contributed to insights into how Ldb1a-mediated complexes govern cell fate determination and tissue patterning in vertebrate models. The antibody’s reliability in zebrafish models underscores its utility in developmental and molecular biology research.
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